摘要
目的:探讨TGF-β1、Smad7与ki-67 mRNA在胃癌中的表达及其相关性。方法:手术切除经病理证实为胃癌组织标本60例,正常胃组织20例,采用RT-PCR技术检测TGF-β1 mRNA、Smad7 mRNA和ki-67mRNA表达水平。结果:TGF-β1、Smad7和ki-67 mRNA在正常胃组织中的相对含量均低于胃癌组织中的表达(P<0.05);三者在高中分化胃癌组织的相对含量均低于低分化胃癌组织(P<0.05);TGF-β1 mRNA、Smad7mRNA和ki-67 mRNA在无淋巴结转移者的表达低于有淋巴结转移者;TNM分期:Ⅰ+Ⅱ期的表达低于Ⅲ+Ⅳ期(P<0.05);TGF-β1 mRNA、Smad7 mRNA、ki-67 mRNA三者在胃癌中的表达经直线相关分析显示呈正相关。结论:TGF-β1、Smad7与ki-67 mRNA的表达与胃癌的发生、发展及分化程度密切相关。
Objective To investigate relationship between the expressions of TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA and the clinicopathologic characteristics in gastric carcinoma. Methods 60 cases with gastric carcinoma tissue and 20 cases with normal gastric tissue, which were surgically removed and certified by pathology, were collected. The expressions of TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA were detected by RT-PCR in normal and carcinomatous gastric tissue. Results The expressions of TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA in normal gastric tissue were lower than those in carcinomatous gastric tissue （P 〈 0.05）. The expressions of TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA in gastric carcinoma which were well differentiated and moderately differentiated were significantly lower than those in poorly differentiated gastric carcinoma （P 〈 0.05 ）. The expressions of TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA in gastric carcinoma without lymph node metastasis were lower than those in gastric carcinoma with lymph node metastasis. The expressions of TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA in I ＋ II stage were lower those in III＋ IV stage （P 〈 0.05 ）. Linear correlation showed there were positive correlations among TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA in gastric carcinoma. Conclusions The canonical TGF-β1 Smad7 and Ki-67 mRNA play a role in the carcinogenesis, development, differentiation of gastric carcinoma.
出处
《实用医学杂志》
CAS
北大核心
2014年第5期751-754,共4页
The Journal of Practical Medicine
基金
河北省2009年医学科学研究重点课题(编号:20090583)
