精子染色质结构分析和染色质扩散试验检测精子DNA碎片的比较研究

Comparison of Sperm Chromatin Structure Assay with Sperm Chromatin Dispersion Test for DNA Fragmentation Evaluation in Human Sperm

目的：探讨精子染色质结构分析（SCSA）和染色质扩散试验（SCD）对精子DNA碎片检测方法的比较及相关性分析。方法：本研究对134例男性不育症患者分别用SCSA和SCD方法进行精子DNA碎片检测，分析两种方法检测结果的相关性。将DNA碎片指数（DFI）分成3个等级组别（≤15％DFI、〉15～≤30％DFI和〉30％DFI），分别比较两种方法检测的结果差异。结果：SCSA方法与SCD方法对精子DNA碎片的分析结果具有明显的线性正相关性（r=0．915，P〈0．0001）。在〉15～≤30％DFI和〉30％DFI两组中．其两种方法分析结果无显著差异。而在≤15％DFI组中，采用SCD方法检测的DFI结果（13．50±4．82）明显高于SCSA方法检测结果（9．79±2．60）（P〈0．001）。结论：SCSA和SCD两种方法检测DNA碎片结果有较好的相关性。在D兀≤15％时。SCD检测结果明显高于SCSA检测结果。

Objective To assess sperm chromatin structure assay （SCSA） and sperm chromatin dispersion test （SCD） for DNA fragmentation evaluation in human infertility, and the correlation between these two methods. Methods We used SCSA and SCD assays to detect DNA fragmentation in sperm from 134 infertile men. The correlation of SCSA and SCD assays was analyzed. The sperm DNA fragmentation index （DFI） was divided into 3 groups （≤ 15%DFI, 〉 15 - ≤ 30%DFI and 〉 30%DFI）, and the difference between SCSA and SCD assays was assessed. Results The SCSA assay was strongly correlated with the SCD assay for sperm DNA fragmentation （r = 0.915, P 〈 0.001）. There was no significant difference between 〉 15 - ≤ 30%DFI and 〉 30%DFI groups. However, SCD showed higher levels of DNA fragmentation than that measured by SCSA for ~〈 15%DFI group （ 13.50 4.82 vs 9.79 2.60, P 〈 0.001 ）. Conclusion There is a strong positive correlation between SCSA and SCD assays in detection of DNA fragmentation. SCD assay showed higher levels of DNA fragmentation than that measured by SCSA for ≤ 15%DFI group.