摘要
采集健康仔猪肠道,从空肠黏膜分离到23株对大肠杆菌和金黄色葡萄球菌有拮抗作用的芽孢杆菌,复筛获得5株拮抗活性较高者;从中筛选出可耐受胆盐、人工胃液和肠液的菌株JN-16,分子生物学和部分生化试验鉴定结果表明:该菌为枯草芽孢杆菌。对菌株JN-16进行抑菌培养条件优化,结果表明,以葡萄糖为碳源、大豆蛋白胨为氮源、pH为6.5的培养基在33℃或37℃下,培养24 h时,发酵液的抑菌效果最强。该菌株可用作饲料添加剂,为微生态制剂的研发奠定了基础。
A total of 23 Bacillus strains antagonistic to Escherichia coli and Staphylococcus aureus were isolated from jejunal mucosa of healthy piglets,and 5 of them possessed higher antagonistic activity.The strain named JN-16 was isolat-ed and tolerant to bile salts,artificial intestinal juice and gastric juice.The results of molecular biology and biochemical ex-periments demonstrated that JN-16 was Bacillus subtilis.The results of antibacterial experiments showed that the antibacte-rial activity of broth was strongest,when the JN-16 was cultured at 33 ℃ or 37 ℃ for 24 h using glucose as carbon source,soy peptone as nitrogen source,pH=6.5.This strain could be used as feed additives,and laid foundation for devel-opment of probiotics.
出处
《中国饲料》
北大核心
2014年第5期21-25,28,共6页
China Feed
基金
绿色环保型猪用饲料添加剂的研发与应用(2011-Y05)
吉林省现代农业产业技术体系建设专项
吉林省安全优质肉猪现代生产关键技术的研究(09ZDGG008)
吉林省科技厅项目(20140204069NY)
关键词
枯草芽孢杆菌
生物学特性
鉴定
条件优化
Bacillus subtilis
bionomics
identification
optimization of conditions
