安徽宣城地区汉族人群18个STR基因座遗传多态性

Genetic polymorphism of 18 STR loci of Han population in Anhui Xuancheng area

目的 调查安徽宣城地区汉族人群5000例无关个体18个STR基因座多态性分布。方法 应用AmpFlSTR Identifiler荧光标记复合扩增系统检测,统计计算群体遗传学参数。结果 18个STR基因座的基因型分布均符合Hardy-Weinberg平衡(P>0.05),共检出270个等位基因,频率在0.0001~0.5260之间,共有1289种基因型,在13个基因座上检出共44个微变异等位基因。18个基因座的杂合度观察值(Ho)在0.6238~0.9120之间,杂合度理论值(He)在0.6961~0.9601之间,多态信息含量(PIC)在0.5578~0.9126之间,累计个人识别率(TDP)为0.999999999999999999999953,三联体累计非父排除概率(CPE)为0.999999993487306,二联体累计非父排除率(CPE*)为0.985819169。结论 本文调查结果与以往文献报道的不同地区民族的人群等位基因频率资料有一定程度的差异性。上述18个STR基因座适用于宣城地区汉族群体各类案件的法医学个体识别和亲权鉴定。

Objective To investigate genetic polymorphism of 18 STR loci of the Han population in Xuancheng area.Methods Allele frequencies of 18 STR loci for 5000 unrelated Chinese individuals in Xuancheng area were determined with Amp-FISTR Identifiler kit and the values of genetic parameters for Forensicusing were calculated.Results No deviations of the ob-served allele frequency from Hardy-Weinberg equilibrium expectations were found for Chi-square test(P>0.05).270 alleles and 1289 genotypes were observed.All ele frequencies varied between 0.0001 and 0.5260.44 off ladder alleles(at 13 STR loci)were found.Observed heterozygosity(Ho)varied between 0.6238 and 0.9120,theoretical heterozygosity(He)varied between 0.6961 and 0.9601,polymorphic information content(PIC)varied between 0.5578 and 0.9126,total discrimination power(TDP)of 18 STR loci was 0.999999999999999999999953,cumulative probability of paternity exclusion(CPE)for triplet cases was 0.999999993487306,CPE for fatherless or motherless cases(CPE*)was 0.985819169.ConclusionThe results showed the 18 STR loci can be used for forensic personal identification and paternity testing.