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香蕉胁迫相关蛋白基因的克隆及其表达分析 被引量:4

Cloning and Expression of Stress Associated Protein Gene from Banana(Musa acuminata L.AAA group,cv.Brazilian)
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摘要 通过随机克隆测序的方法从香蕉根系cDNA文库中获得胁迫相关蛋白基因,命名为MaSAP1(GenBank登录号为AGH14257.1)。扩增获得的cDNA序列与质粒OZ092的目的片段序列一致,表明MaSAP1是香蕉SAP基因编码框全长cDNA,包含一个510bp的最大开放阅读框,编码一个长169个氨基酸的蛋白质。蛋白质序列同源比对发现其含有完整的A20和AN1基序结构。系统进化树比对分析表明,MaSAP1与水稻和獐茅的亲缘关系较近。组织特异性研究表明,MaSAP1基因在香蕉根和果实中的表达量较高,在茎中的表达量最低。实时荧光定量PCR分析表明MaSAP1响应激素的处理,同时也响应干旱、低温、高盐和枯萎病菌的侵染等胁迫。可见,MaSAP1基因在植物生长发育和植物响应逆境中具有重要作用。 The aim of this paper is to acquire genes related to the stress response in banana. A stress associ- ated protein gene was acquired from banana roots cDNA library by randomly sequencing,which was named MaSAP1 (GenBank accession number: AGH14257. 1). The sequence amplified from banana root cDNA was the same with fragment sequences of plasmid OZ092 in library, indicating that MaSAP1 was a full- length cDNA,containing a 510 bp of the largest open reading frame,encoding 169 amino acids. The homolo- gy alignment of protein sequence found to contain complete A20 and AN1 motif. Phylogenetic analysis indi- cated that the MaSAP1 was closer with Oryza sativa and Aeluropus littoralis. Tissue-specific expression study showed that the expression of MaSAP1 was higher in the roots and fruits,the lowest in the stems. Real-time PCR analysis showed MaSAP1 in response to hormone treatment, and also in response to various stress, such as drought,low temperature, high salt and FocTR4. Altogether the results in this work demon- strated that MaSAP1 may play an important biological role in plant growth and development,and plant re- sponse to different stress types.
出处 《西北植物学报》 CAS CSCD 北大核心 2014年第2期225-230,共6页 Acta Botanica Boreali-Occidentalia Sinica
基金 国家自然科学基金项目(31071788) 国家现代农业产业体系项目(CARS-32)
关键词 香蕉 胁迫相关蛋白 基因克隆 表达 banana stress associated protein gene clone expression
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