基于ITS和trnL-trnF序列的草木樨种群遗传多样性研究

Genetic Diversity Analysis of Melilotus Populations Based on ITS and trnL-trnF Sequences

以白花草木樨(Melilotus alba)和黄花草木樨(Melilotus officinalis)18个地理种群植物为材料,用ITS序列和trnL-trnF序列研究了2种草木樨不同种群间的遗传多样性。结果表明:(1)trnL-trnF序列对位后长度为459bp,其中包括6个变异位点,6个简约信息位点,G+C含量为33.1%;ITS序列对位后长度为714bp,其中包括5个变异位点,3个简约信息位点,G+C含量为48.9%。(2)在基于trnL-trnF序列构建的系统发育树中,2种草木樨能够形成单系分支,说明trnL-trnF序列在草木樨中的鉴别能力较强。(3)单倍型多样性以及核苷酸多样性分析表明,黄花草木樨的遗传多样性高于白花草木樨。

Eighteen populations of Melilotus alba and Melilotus officinalis were used to study the genetic diversity by ITS and trnL-trnF sequences. The results showed as followings： （1）The aligned length of trnL-trnF sequences was 459 bp and there were 6 parsimonious informative sites, 6 variable sites and 33.1% G＋C content. The aligned length of ITS sequence was 714 bp and there were 4 parsimonious in- formative sites,6 variable sites and 48.9% G-＋-C content. （2）All Melilotus L. populations could be divided into two groups in the phylogenetic tree of trnL-trnF,which showed that trnL-trnF sequence had the iden- tify ability in the molecular phylogeny study of Melilotus L.. （3）The genetic diversity of M. officinalis was higher than that of M. alba in both sequences by analyzing haplotypes and nucleotide diversity.