Sox1抑制膀胱癌增殖及转移的作用及分子机制研究

Sox1in inhibiting proliferation and metastasis of bladder cancer and its molecular mechanisms

目的:研究Sox1抑制膀胱癌增殖及转移的作用及分子机制。方法:通过慢病毒系统在T24细胞中上调Sox1的表达,在激光共聚焦显微镜下观察其结果,并通过相关实验观察其反应。结果:激光共聚焦显示Sox1定位于细胞核内,可显著抑制肿瘤细胞增殖能力;细胞侵袭实验表明Sox1可显著抑制细胞的迁移和侵袭能力;荧光素酶报告基因结果显示Sox1可以抑制β-catenin转录活性。结论:Sox1是重要的抑癌基因。本研究揭示了膀胱癌发生及转移的新机制,加深了对膀胱癌发生和转移的认识。

Objective： To study the role of Soxl in inhibiting proliferation and metastasis of bladder cancer and its molecular mechanisms. Method： We employed lentivirus to elevate expression of Soxl. Such results were con- firmed by confocal laser scanning microscope. Relevant tests were taken to observe the reaction. Result： Confocal laser scanning microscope showed that Soxl was located in the nucleus. It was also shown that Soxl was able to significantly inhibit the proliferation of cancer cells. The ability of Soxl in inhibiting cell migration and invasive was able to be seen in cell invasion assay. Luciferase reporter assay showed that overexpression of Soxl sup- pressed transcription of β-catenin. Conclusion： Soxl has been regarded as an key anti-oncogene. This study provides a new insight into the molecular mechanisms leading to carcinogenesis and has deepened our knowledge of bladder cancer.