原发性肝癌患者中血清miR-1的表达及意义

Expression of serum miR-1 and its clinical significance in primary hepatic carcinoma

目的探讨原发性肝癌（PHC）患者血清miR-1的表达及其与临床病理特征之间的关系。方法采用实时定量PCR检测90例PHC患者及102例健康志愿者血清中miR-1的表达量。结果PHC组中miR-1表达量[0．210（0．127～0．312）]明显低于健康对照组[0．780（0．618～1．121）]及PHC术后7d组[0．540（0．318—0．716）]（P〈0．001）。在90例PHC患者中，miR-1表达水平与淋巴结有无转移密切相关，有淋巴结转移患者中miR-1表达水平显著低于无淋巴结转移患者（P=0．003）。Kaplan—Meier分析显示miR-1低表达组的生存率远低于其高表达组，其中位生存期（月）分别为25．48和40．98（P〈0．001）。Cox多变量分析显示miR-1水平是影响PHC患者生存率的独立危险因素之一，相对危险度为0．424（95％CI：0．183—0．984）。结论血清miR-1作为新的生物学指标对于PHC的诊断和预后的具有一定的价值。

Objective To investigate the serum expression level of miR - 1 in patients with primary hepatic carcinoma （PHC） and its clinical significances. Methods The serum expression levels of miR - 1 in 102 healthy controls and 90 PHC patients were measured by real -time fluorescent quantitative -polymerase chain reaction （qRT- PCR）. Results Serum miR - 1 ex- pression level in PHC pre - operative group[0. 210（0. 127 - 0. 312） ] was significantly lower than that in the control group [ 0. 780 （0.618 ± 1. 121 ） ] and the post - operative group [ 0.540 （0.318 ± 0.716 ） ] （ P 〈 0.001 ）. Serum miR - 1 expression level was far lower in PHC patients with lymph node metastasis than those without, showing close correlation between miR - 1 expression level and lymph node metastasis （ P = 0.003 ）. Kaplan - Meier curve analysis revealed that PHC patients with a low serum miR - I expression level exhibited significant short survival time when compared to those with a high serum miR - 1 ex- pression level （25.48 months vs 40.98 months, P 〈 0. 001 ）. Multivariable Cox regression analysis indicated that the serum miR - 1 expression level was an independent risk factor for survival rate of PHC patients. Relative risk was 0.424 （95% confi- dence： 0. 183 - 0. 984）. Conclusion As a new biological marker, serum miR - 1 is helpful to diagnose PHC and judge the progression and prognosis of the disease.