乙型肝炎病毒感染模式与HBV-DNA的相互关系

The interrelation of hepatitis B virus infection model and HBV DNA

目的分析4种最常见乙型肝炎病毒感染模式与HBV-DNA载量的相互关系。方法用ELISA法和FQ-PCR技术对乙型肝炎患者分别进行血清学模式及HBV-DNA载量检测。结果 ELISA法4种模式患者HBV-DNA阳性率分别为:94.1%、96.6%、33.6%、43.2%。阳性率比较:①HBeAg阳性组与HBeAg阴性组比较(P<0.05);②HBeAg阳性组与HBeAg阳性组比较(P>0.05);③HBeAg阴性组与HBeAg阴性组比较(P>0.05);④大三阳组(Ⅰ)与小三阳组(Ⅲ)比较(P<0.05)。HBV-DNA病毒载量分布:HBeAg阳性组与HBeAg阴性率组HBV-DNA病毒载量103copy/ml^105copy/ml、105copy/ml^107copy/ml、≥107copy/ml 3个水平两两比较:(P<0.05)。结论不同的HBV-M与HBV-DNA载量存在相关性,两种检测方法结合对乙型肝炎的诊断、治疗、预后有重要的临床价值。

Objective To analyze the interrelation between hepatitis B virus infection model（ HBV - M） and HBV - DNA load. Methods ELISA method and FQ - PCR technique were employed to detect the serum markers and HBV - DNA load, respec- tively. Results By ELISA, the positive rates of HBV - DNA in patients of four different infection modes were 94. 1%, 96.6%, 33.6% and 43.2% respectively. The positive rates comparison results were as follows, HBeAg positive group vs HBeAg negative gronp（P 〈 0.05 ）, HBeAg positive group vs HBeAg positive group（P 〉 0.05 ）, HBeAg negative group vs HBeAg negative group（P 〉 0. 05 ）, HBsAg- HBeAg- HBeAb positive（ I ） group vs HBsAg- HBeAb -HBcAb positive （ III ） group （ P 〈 0. 05 ）. The distribution of HBV - DNA viral load was analyzed by pairwise comparison of HBeAg positive group and HBeAg negative group at HBV - DNA load levels of 103 copy/ml - 10^5 copy/ml, 105 eopy/ml - 10^7 copy/ml, ≥ 10^7 copy/ml（P 〈 0. 05）. Conclusion There is correlation between HBV - M and HBV - DNA load. Combination of ELISA and FQ - PCR has important clinical value in the diagnosis, treatment and prognosis of hepatitis B.