深圳地区CTLA4基因SNPS与儿童哮喘易感性研究

Study on the association between CTLA4 genes SNPS and pediactric asthma susceptibility in Shenzhen

目的初步探讨哮喘易感基因CTLA4单核苷酸多态性(-318C/T、+49 A/G、-443 A/T、-658C/T、-994A/G、-1147C/T SNP位点)在深圳哮喘患儿中的基因频率和基因型频率。方法分别收集深圳地区40例哮喘患儿及正常儿童外周血标本,应用基因测序技术对-318C/T、+49 A/G、-443 A/T、-658C/T、-994 A/G、-1147C/T SNP位点进行研究,分析在深圳地区儿童中其多态性、基因频率与哮喘之间的关系。结果在哮喘组和对照组中,-318C/T、-443 A/T、-1147C/T位点多态性、基因频率无明显差异(P>0.05);+49 A/G、-658C/T、-994 A/G位点基因型及基因频率均有显著性差异。结论在深圳哮喘儿童中+49 A/G、-658C/T、-994 A/G位点多态性可能发生的频率较高,-318C/T、-443 A/T、-1147C/T位点多态性的发生频率较低。

Objective To investigate the gene frequency and genotype frequency of CTLA4 SNPS（ - 318C/T SNP, ＋ 49 A/ G SNP, -443 A/T SNP, - 658C/T SNP, - 994 A/G SNP, - 1147C/T SNP） in asthma children in Shenzhen. Methods CTLA4 - 318C/T SNP, ＋ 49 A/G SNP, -443 A/T SNP, - 658C/T SNP, - 994 A/G SNP, - 1147C/T SNP were analyzed by DNA sequencing in 40 ehildren with asthma and 40 healthy children in Shenzhen. Results The gene frequency of -- 318C/T, -443 A/T, - 1147C/T SNPS of the CTLA4 gene had no obvious differenee between the asthma children group and the control group（P 〉0.05）. While that of ＋49A/G, - 658C/T and - 994 A/G of the CTLA4 gene had obvious differ- enee between the asthma children group and the eontrol group. Conclusion The frequeney of - 318C/T, -443 A/T and - 1147C/T SNPS of the CTLA4 gene is low in children with asthma in Shenzhen, but that of the ＋ 49 A/G, - 658C/T , - 994 A/G SNPS is higher.