654例急性腹泻患者中致泻性病毒病原学研究

Etiological study on viral pathogens in 654 acute diarrhea cases

目的了解最近两年衢州地区急性腹泻患者中病毒的病原学分布特点。方法收集2011年-2012年衢州地区654例急性腹泻患者的粪便标本和流行病学资料,并进行腹泻相关病毒的多重RT-PCR和PCR检测。结果急性腹泻患者病毒检出率为19.11%(125/654),其中轮状病毒(RV)A型54例(8.26%)、C型3例(0.46%),诺如病毒(NV)GⅠ型6例(0.92%)、GⅡ型28例(4.28%),札如病毒(SAV)31例(4.74%),星状病毒(ADV)3例(0.46%);未检测到肠道腺病毒(ASV)。轮状病毒主要感染年龄为≥18岁,其他病毒在不同年龄组人群中感染率差异无统计学意义。轮状病毒发病高发期在11月至次年1月,杯状病毒的感染主要集中在2月-6月。结论衢州地区急性病毒性腹泻的病原复杂,杯状病毒是最主要病原。不同病种类病毒感染具有明显的季节性,制定防治策略时应予以重视。

Objective To investigate the etiology characteristics of virus in acute diarrhea patients in Quzhou from 2011 to 2012. Methods Epidemiologie data and fecal specimens were collected from 654 patients with acute diarrhea. Diarrhea - re- lated virus were detected by multiple RT - PCR and PCR. Results The virus detection rate was 19.11% in acute diarrhea pa- tients（125/654）, including rotavirus A in 54 eases（8.26% ）, rotavirus C in 3 eases（0.46% ）, norovirus G I in 6 eases （0.92%）, norovirus G lI in 28 casses（4.28% ）, sapovirus in 31 eases（4.74% ）, and astrovirus in 3 eases（0.46% ）. No enteric adenovirus was found. In addition, the incidence of rotavirus was highest in people more than 18 years old. There was no significant difference in other viruses among different age groups. The peak of rotavirus diarrhea was observed from October to January of the next year, while human calieiviruses infection mainly concentrated from February to June. Conclusion Human ealicivirus was the most important pathogen of acute diarrhea among muhiple virus in Quzhou. The occurrence of different virus has obvious seasonal features, which should be given enough attention in control and prevention of acute viral diarrhea.