运动训练对大鼠植物神经心率调节功能的影响

The Effect of Exercise on Heart Rate Regulation of Visceral Nerve in Rats

目的:通过运动能力测试,建立不同运动强度训练的大鼠跑台运动模型,探讨不同运动强度对大鼠植物神经心率调节功能的影响。方法:10周龄雄性SD大鼠27只,经最大运动速度测试之后,随机将大鼠分为3组,即:空白对照组、中等强度运动组以及大强度运动组,各组大鼠不同运动强度训练8周后,测试大鼠安静状态下心率、血压,植物神经阻断后的变化心率以及大鼠右心室心房M2受体、RGS6以及KCNJ5的含量;结果:1)硫酸阿托品能够显著性增加中等强度及大强度运动训练大鼠心率,但盐酸普莱洛尔的影响不大;2)中等强度运动训练显著性增加M2受体含量,但两种运动方式对RGS6和KCNJ5含量没有影响;3)中等强度及大强度运动均能够降低M2受体mRNA、RGS6mRNA及Kir3.4mRNA的含量;结论:8周中等强度运动以及大强度运动训练对安静心率影响不大;但能够通过调节迷走神经纤维自身功能,从而有效降低正常大鼠迷走神经心率调节能力,对迷走神经之后的心内调节通路蛋白及交感神经心率调节功能影响不大。

Objective: This paper aimed to establish different exercise model of intensity by exercise tests,and to detect the effects of different intensity exercise on visceral nerve to regulate heart rate in rats. Method: After maximum exercise capacity tests,twenty-four ten weeks old male SD rats were divided into three groups: the control group( C),middle-intensity exercise group( M),and high-intensity exercise group( H). Rats in M and H groups took 8 weeks training. The rest heart rate,blood pressure,the heart rate after blocking visceral nerves,and the volumes of M2 receptor,RGS6,KCNJ5 of all the rats were measured after 8 weeks. Results: 1) The heart rate of rats in M and H groups was significantly increased after injecting atropine sulfate,but not happened after injecting propranolol hydrochloride; 2) The volume of M2 receptor was increased in M group,but the volumes of RGS6 and KCNJ5 didn't change in both exercise groups( M and H); 3) The mRNA expressions of M2,RGS6 and KCNJ5 were decreased after middle-intensity exercise and high-intensity exercise. Conclusion: Eight weeks mid-intensity exercise and high-intensity exercise can regulate the function of vagus nerve although they didn't influence heart rate. They can reduce the vagus nerve function to regulate heart rate,but they have no effect on proteins in pathways after vagus nerves effects,and have no effect on heart rate regulation of sympathetic.