白术七物颗粒对结肠慢传输型便秘小鼠肠道传输功能的影响

Effect of Baizhu Qiwu granules on intestinal transit function in mice with colonic slow transit constipation

目的:研究中药白术七物颗粒对结肠慢传输型便秘小鼠肠道传输功能的影响。方法:将60只昆明种小鼠随机分为正常组(15只)和模型组(45只)。模型组小鼠皮下注射盐酸吗啡2.5mg·kg-1·d-1建立结肠慢传输型便秘(STC)模型,正常组皮下注射等量等渗生理盐水,共45d。将造模成功的40只小鼠随机分为3组:对照组(10只)、白术七物颗粒组(15只)和莫沙必利组(15只)。各组小鼠分别予以相应药物灌胃治疗,正常组和对照组小鼠予等量等渗生理盐水灌胃,每天1次,连续14d。于给药第14天计算每组小鼠当天粪便干重,末次给药后测定并对比分析各组小鼠肠道墨汁推进率。结果:治疗后各治疗组小鼠粪便干重均有不同程度增加,肠道墨汁推进率提高,且白术七物颗粒组改善程度优于莫沙必利组,差异有统计学意义(P<0.05)。结论:白术七物颗粒能增强结肠慢传输型便秘小鼠的肠道传输功能。

Objective ：To investigate the effect of Baizhu Qiwu granules, a traditional Chinese medicine preparation, on the intestinal transit function in mice with colonic slow transit constipation （CSTC）. Methods ： Sixty Kunming mice were randomly divided into normal group （n = 15 ） and model group （n = 45 ）. The model group was subcutaneously injected with morphine hydrochloride （2. 5 mg/kg/d） for 45 d to establish a CSTC model, while an equal volume of isotonic saline was given instead of morphine hydrochloride in the normal group. The 40 model mice were randomly divided into control group （ n = 10 ）, Baizhu Qiwu granule group （ n = 15 ）, and mosapride group （n = 15 ）. The Baizhu Qiwu granule group and mosapride group were given respective drugs once daily for 14 d,while the normal group and control group were given an equal volume of isotonic saline instead. The dry weight of feces in each group on day 14 of treat- ment was calculated. After the final administration, the colon ink propelling rate was determined and compared between all groups. Results：After treatment,the Baizhu Qiwu granule group and mosapride group showed variable increases in dry weight of feces and colon ink propelling rate, and the Baizhu Qiwu granule group had significantly higher increases than the mosapride group （P 〈 0. 05）. Conclusion：Baizhu Qiwu granules can improve the intestinal transit function in mice with CSTC.