豌豆核基质结合区的功能研究

Study on Activity of Pea MAR

将豌豆基因组中分离的核基质结合区 (Matrixattachmentregions ,MARs)构建在植物表达载体的T DNA结构域中 ,使得报导基因 β 葡糖醛酸酶 (β glucuronidase ,GUS)基因位于两段MARs序列之间。将此载体与不包含MARs序列的植物表达载体经农杆菌介导转化烟草。GUS活性检测表明 ,MARs可以提高GUS基因的表达水平 ,和不包含MARs的转基因植株相比 ,MARs序列使GUS基因的平均表达水平提高 2倍。但在瞬时表达中 ,MARs对GUS基因的表达没有影响。

The T DNA construction containing pea matrix attachment regions was constructed, in which β glucuronidase GUS gene were flanked by the MARs. The plant expression vectors with and without MARs were transferred into tobaccos via Agrobacterium mediated transformation procedure. The results of GUS activity showed that MARs could increase levels of GUS gene expression, the mean GUS activity could be increased two fold compared with that of transformants without MARs. But MARs had no effect on the GUS gene expression levels in transient expression.