小白鼠心肌核DNA片段中的基因表达的分子开关的研究

The Study on the Molecular Switch of Gene Expression of the Mouse Heart Nuclear DNA Fragments

原位染色发现 ,LDH( 1- 5) …nNAD+(LDH :乳酸脱氢酶 ,NAD+:氢化型辅酶I)可以进入核孔 ,可能与自己编码基因特异结合。体外表达实验中 ,稀释心肌DNA片段 ,促进LDH/DNA 表达LDH( 1- 5) ,LDH( 1- 5) 表达量与LDH/DNA 中可解离的LDH( 1- 5) 量呈正相关性。同位素14 C标记Leu法测定加入LDH( 1- 5) …nNAD+可以抑制LDH/DNA 体外表达 ,LDH( 1- 5) 表达抑制量与加入LDH( 1- 5) …nNAD+量呈正相关性。AFM直接观察到活性基因两头的调控序列可能结合自己编码的蛋白等活性因子。基因表达与调控的分子开关主要是自己编码的蛋白等活性因子。

It is observed by in situ stain that LDH (1-5) …nNAD + can probably enter the nucleopore and bind specifically with the gene that encodes itself. During the in vitro expression, the dilution of heart nuclear DNA fragments could enhance the expression activity of LDH/DNA*, the amount of expressed LDH (1-5) is in proportion to the amount of dissociable LDH (1-5) on the LDH/DNA*. With the integration of the 14 C-Leu to the protein, it is also observed that the addition of LDH (1-5) …nNAD + may suppress the in vitro expression activity of LDH/DNA* and the decreased amount of expressed LDH (1-5) is in proportion to the amount of added LDH (1-5) …nNAD +. AFM observation shows that the regulation sequence at the two ends of the active genes may bind with such active factors as the proteins encoded by the genes themselves.