南美斑潜蝇幼虫为害对黄瓜叶片中水杨酸和茉莉酸的诱导作用

Induction of salicylic acid and jasmonic acid in cucumber leaves after infestation by Liriomyza huidobrensis( Diptera: Agromizidae) larvae

【目的】为揭示南美斑潜蝇Liriomyza huidobrensis(Blanchard)与其寄主相互作用的机理,为利用诱导抗性控制南美斑潜蝇的发生为害奠定必要的基础。【方法】本文采用高效液相色谱法(HPLC)和超高效液相色谱法-质谱联用法(UPLC-MS),分别测定了南美斑潜蝇幼虫为害对黄瓜叶片中茉莉酸(jasmonic acid,JA)和水杨酸(salicylic acid,SA)的诱导作用。【结果】南美斑潜蝇幼虫持续为害1 d后,受害黄瓜叶片内JA含量即显著高于健康对照,轻度受害处理和重度受害处理分别在第3天和第5天上升幅度最大,分别比健康对照增加2.01倍和1.62倍;而SA含量在3 d后才显著高于健康对照,轻度受害处理和重度受害处理在第9天上升幅度最大,分别比健康对照增加4.66倍和1.67倍;轻度受害对JA和SA的系统诱导作用不明显,而重度受害对JA和SA具有明显的系统诱导作用。【结论】南美斑潜蝇幼虫为害对黄瓜叶片内JA和SA具有诱导作用。

[ Aim] In order to investigate the mechanism of interactions between Ltrtomyza ttutctobrensts （Blanchard） and its host plants and build an indispensable foundation for controlling the fly by use of inducible defenses. [ Methods] High-performance liquid chromatography （HPLC） and ultra-performance liquid chromatography-mass spectrometry （UPLC-MS） were used to determine the effects of infestation of larval L. huidobrensis on induction of jasmonic acid （JA） and salicylic acid cucumber leaves. [ Results ] The results showed that JA contents in the damaged （ SA）, respectively, in leaves were significantly higher than those in intact controls only 1 d after the larval continuous infestation, and the increase magnitudes in the light-damage treatment and the severe-damage treatment reached the maximum at 3 -5 d after larval infestation, increasing 2. 01 and 1. 62 times as compared with the intact control, respectively. SA contents were significantly higher at 3 d after larval infestation, and the increase magnitudes in the light-damage treatment and the severe-damage treatment reached the maximum at 9 d after larval infestation, rising 4.66 and 1.67 times as compared with the intact control, respectively. The induction of JA and SA was significantly systemic in the severe-damage treatment but not in the light- damage treatment. [ Conclusion ] The results suggest that the larval infestation of L. huidobrensis can induce the JA and SA increase in cucumber leaves.