氟保护漆抑制酸性含乳饮料对乳牙釉质脱矿的作用

Study of the effect of fluor protector on resistance to demineralization of milk beverages on enamel of primary teeth

目的:研究氟保护漆应用于乳牙釉质表面对酸性含乳饮料致离体乳牙釉质脱矿的抑制作用,阐明氟保护漆的抗脱矿作用,为氟保护漆的临床推广及婴幼儿群防提供实验室依据。方法:将预备好的30个无龋、无明显裂纹及釉质发育缺陷的乳牙唇侧釉质块随机分为人工唾液A组(对照组)、B组(酸性含乳饮料组)、C组(0.1%氟保护漆组),每组10个标本。C组标本之釉质表面用小毛刷均匀涂布氟保护漆2次,自然晾干45 min。A组置于37℃恒温的10 mL人工唾液中浸泡4 d。B、C 2组在37℃恒温水箱中浸泡于10 mL酸性含乳饮料中,浸泡2 min,取出1 min,再浸泡,共9 min。3次/d,共4 d。3组牙釉质制作标本,应用场发射扫描电镜和能谱分析仪,检测乳牙釉质的表面形态变化,Ca2+、P3+含量数值(重量百分比)及Ca2+、P3+比值,采用SPSS 11.5软件包对数据进行统计学分析。结果:酸性含乳饮料浸泡可致乳牙釉质Ca2+、P3+含量显著降低(P<0.01)。氟保护漆处理后的釉质Ca2+、P3+含量降低程度显著低于酸性含乳饮料浸泡组(P<0.01)。结论:酸性含乳饮料对乳牙釉质表面有较强的酸蚀脱矿作用。釉质表面应用氟保护漆可增加硬度,在一定程度上抑制乳牙釉质在酸性含乳饮料中的脱矿,但不能完全抵御饮料的酸蚀。

PUPPOSE： To study the effect of fluor protector against demineralization of deciduous teeth enamel surface in milk beverages, to certify the mechanism of anti-demineralization of fluor protector, and provide laboratory basis for clinical practice of fluor protector and the prevention of infants and young children. METHODS： The enamel surfaces of 30 prepared deciduous teeth without caries were randomly divided into 3 groups. Group A was control group. Milk beverages were used in group B, and 0.1% fluor protector was used in group C. Each group included 10 specimens. All specimens were soaked in water tank at 37~C for 4 days, but specimens in groups B and C were soaked at intervals in 10mL yogurt drinks in water tank at 37~C, 9 minutes for one time and 3 times for one day. Scanning electron microscope （SEM） and spectrum analyzer were used to detect the changes in surface morphology of deciduous teeth enamel surface. The concentration values and the ratio of calcium / phosphorus were analyzed with SPSSll.5 software package. RESUITS： Enamel soaked in milk beverages had decreased Ca2 ＋and p3 ＋ content significantly （P〈0.01）. Ca2 ＋and p3 ~ content in enamel in group C was significantly higher than in group B （P〈0.01）. Scanning electron microscopy showed enamel surface in group C had mild demineralization. CONCLUSIONS： Milk beverages can cause a strong acid demineralization in deciduous teeth enamel surface. The application of fluor protector in enamel surface can inhibit enamel demineralization in the milk beverages.