期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

调节性T细胞与Th17细胞相关因子在实验性自身免疫性肌炎小鼠淋巴结中的表达及意义 被引量：8

下载PDF
导出
摘要 目的研究调节性T细胞(Treg)、Th17细胞的相关细胞因子在实验性自身免疫性肌炎小鼠淋巴结中表达的作用及意义。方法雌性BALB/c小鼠12只,随机分为正常对照组和模型组,模型组采用肌球蛋白诱导实验性自身免疫性肌炎(EAM)。采用实时荧光定量PCR(qRT-PCR)检测各组小鼠淋巴结中与Treg和Th17细胞分化及功能相关的细胞因子Foxp3、IL-10、TGF-β、RORγt、IL-6、IL-17、IL-23的mRNA表达。结果与正常小鼠相比,肌炎组小鼠淋巴结中Treg的转录因子Foxp3表达降低,相关的细胞因子IL-10、TGF-βmRNA表达水平增高;Th17的转录因子RORγt及相关的细胞因子IL-6、IL-17、IL-23 mRNA表达有不同程度的升高(P<0.05)。结论实验性自身免疫性肌炎中Treg表达降低,而Th17及其相关因子表达升高,提示Treg和Th17之间的失衡在肌炎发病机制中起一定作用。
作者 何方园 张红鸭 胡萌萌 张雅茜 贾宏阁
机构地区 第四军医大学西京医院神经内科
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2014年第4期399-402,共4页 Chinese Journal of Cellular and Molecular Immunology
基金 国家自然科学基金(81171181)
关键词 实验性自身免疫性肌炎 调节性T细胞 TH17细胞 细胞因子
分类号 R593.2 [医药卫生—内科学] R392.11 [医药卫生—免疫学]
  • 相关文献

参考文献2

二级参考文献121

  • 1Dalakas MC, Hohlfeld R. Polymyositis and dermatomyositis[ J]. Lancet, 2003, 362(9388): 971-982.
  • 2Allenbach Y, Solly S, Gre 'goire S, et al. Role of regulatory T cells in a new mouse model of experimental autoimmune myositis [ J ]. Am J Pathol, 2009, 174(3): 989-998.
  • 3Jonuleit H,Sehmitt E,Stasen M,et al.Identification and functional characterization of human SD4^+CD25^+T ceils with regulatory properties isolated from peripheral blood[J].J Exp Med,2001,193(11):1285-1294.
  • 4Fontenot JD, Gavin MA, Rudensky AY. Foxp3 programs the development and function of CD4^+ CD25^+ regulatory T cells[ J]. Nat Immunol, 2003, 4(4): 330-336.
  • 5Matsumoto Y, Kohyama K, Park IK, et al. Characterization of pathogenic T cells and autoantibodies in C-protein-induced autoimmune polymyositis [ J]. Neuroimmunol, 2007, 190 ( 1 - 2) : 90 - 100.
  • 6Venken K, Hellings N, Thewissen M, et al. Compromised CD4^+ CD25^highghregulatory T-cell function in patients with relapsing-remitting multiple sclerosis is correlated with a reduced frequency of FOXP3- positive cells and reduced FOXP3 [J]. Immunology, 2008, 123(1 ) expression at the single cell level 79 - 89.
  • 7Mosmann TR, Coffman RL. TH1 and TH2 cells: different patterns of lymphokinesecretion lead to different functional properties. Annu Rev Immunol 1989; 7: 145-173.
  • 8Korn T, Bettelli E, Oukka M, Kuchroo VK. IL-17 and Th17 Cells. Annu Rev Immunol2009; 27: 485-517.
  • 9Zhu J, Yamane H, Paul WE. Differentiation of effector CD4 T cell populations. AnnuRev Immunol 2010; 28: 445-489.
  • 10O’Shea JJ, Paul WE. Mechanisms underlying lineage commitment and plasticity ofhelper CD41 T cells. Science 2010; 327: 1098-1102.

共引文献29

同被引文献136

  • 1Femando V, Omura S, Sato F, et al. Regulation of an autoimmune model for multiple sclerosis in Th2-biased GATA3 transgenic mice [J]. Int J Mol Sci, 2014, 15(2) : 1700 -1718.
  • 2Geremia A, Jewell DP. The IL-23/IL-17 pathway in inflammatory bowel diseases [ J ]. Expert Rev Gastroenterol Hepatol, 2012, 6 (2) : 223 - 237.
  • 3Yin H, Li X, Zhang B, et al. Sirolimus ameliorates inflammatory responses by switching the regulatory T/T helper type 17 profile in routine colitis[J]. Immunology, 2013, 139(4) : 494 -502.
  • 4Geremia A, Biancheri P, Allan P, el al. Innate and adaptive immunity in inflammatory bowel disease[ J]. Autoimmun Rev, 2014, 13 (1) : 3 - 10.
  • 5Xiao J, Liu C, Li G, el al. PDCD5 negatively regulates autoimmunity by upregulating FOXP3 + regulatory T cells and suppressing Thl7 and Thl responses[ J]. Autoimmun, 2013, 47 : 34 -44.
  • 6Stronmes IM, Govenm JM. Active nduction af experimental allergic encephalomyelitis [ J ]. Nat Protoc, 2006, 4 ( 1 ) : 1810 - 1819.
  • 7Lan Q, Fan H, Quesniaux V, et al. Induced Foxp3 regulatory T cells: a potential new weapon to treat autoimmune and inflammatory diseases[J]? Mol Cell Biol, 2012, 4(1) : 22-28.
  • 8Ohkumn N, Kitagawa Y, Sakaguchi S. Development and maintenance ff regulatory T cells[J]. Immunity, 2013, 38(3) : 414 -423.
  • 9Chen Z, Lin F, Gao Y, et al. FOXP3 and RORt: transcriptional regulation of Treg and Th17 [ J]. Int Immunopharmacol, 2011, 11(5) : 536 -539.
  • 10Cumtto DE, Lafaille MA, Lafai]le JJ. Natural and adaptive Foxp3 regulatory T cell: more of the same or a division of labor [ J ]. Immunity, 2009, 30 (5) : 626 - 635.

引证文献8

二级引证文献33

细胞与分子免疫学杂志
2014年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部