非小细胞肺癌组织中低表达microRNA表达谱

Low expression profiling of microRNA in non-small cell lung cancer tissues

目的:检测非小细胞肺癌患者手术标本组织中低表达microRNA的表达谱,为进一步阐述肿瘤的发生发展机制和提供诊断或治疗的新策略提供基础,积累以国内患者为数据来源的microRNA资料。方法:利用芯片技术初步筛选在非小细胞肺癌组织中低表达microRNA,进而利用实时荧光定量PCR验证癌组织和正常肺组织表达水平的差异。结果:芯片结果发现有20种microRNA在非小细胞肺癌组织中发生明显下调。实时荧光定量PCR验证发现其中的13种microRNA表达量显著降低,分别是hsa-miR-125a-5p、hsa-miR-143、hsa-miR-519a、hsa-miR-223-5p、hsa-miR-1、hsa-miR-520c-3p、hsa-miR-489、hsa-miR-27a、hsa-miR-373、hsa-miR-125b、hsa-miR-27b、hsa-miR-142-5p、hsa-miR-206。结论:在非小细胞肺癌组织中发现一些表达水平显著下调的mciroRNA,这些microRNA可能与肿瘤的发生发展或某些功能相关。

Objective： To describe the expression profiling of microRNAs of patients with non-small cell lung cancer. Methods： We reduced the scope of downregulated microRNAs in patients with non-small lung cancer by high throughput microarray based on quantitative real-time PCR （qRT-PCR）. In this process, the downregulated microRNAs of non-small cell lung cancer tissues can be de- tected and compared with adjacent healthy lung tissues with relatively small samples. Different microRNA expression levels in non-small cell lung cancer and adjacent healthy lung tissues were verified in a large sample by RT-PCR. Results： A total of 20 types of microRNAs were significantly downregulated in non-small cell lung cancer tissues compared with adjacent healthy lung tissues. These mieroRNAs were listed as follows： hsa-miR-1; hsa-miR-22; hsa-miR-27a; hsa-miR-27b; hsa-miR-125a-Sp; hsa-miR-125b; hsa-miR-142-5p; hsa-miR-143; hsa-miR-148a; hsa-miR-148b; hsa-miR-370; hsa-miR-373; hsa-miR-381; hsa-miR-489; hsa-miR-519a; hsa-miR-376c; hsa-miR-206; hsa-miR-380-Sp; hsa-miR-223-Sp; and hsa-miR-520c-3p. Among these microRNAs, 13 types were down- regulated in non-small cell lung cancer tissues as verified by RTPCR. These 13 types of microRNAs were listed as follows： hsa-miR-125a-5p; hsa-miR-143; hsa-miR-519a; hsa-miR-223-5p; hsa-miR-1; hsa-miR-520c-3p; hsa-miR-489; hsa-miR-27a; hsa-miR-373; hsa-miR-125b; hsa-miR-27b; hsa-miR-142-Sp; and hsa-miR-206. Conclusion： In non-small lung cancer tissue, several kinds of microRNAs were significantly downregulated. Changes in microRNA expressions were significantly associated with tumori- genesis, progression, or other cancer cell functions in non-small cell lung cancer.