摘要
目的:探讨组蛋白乙酰化修饰与RAR-β2表达及宫颈病变发生发展的关系。方法:收集正常宫颈、宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)Ⅱ-Ⅲ和宫颈鳞状细胞癌(squamous cell carcinoma,SCC)组织,分别采用免疫组织化学和WesternBlot方法检测AcH3、RAR—B:和Involucrin的表达;分析组蛋白乙酰化水平与RAR-β2表达及宫颈病变程度的关系。结果:随着宫颈病变的进展,AcH3、RAR-β2和Involucrin的表达逐渐降低,甚至缺失,组间差异有统计学意义(P〈0.05);组蛋白乙酰化水平与RAR-β2表达呈正相关(r=0.797,P〈0.05),AcH3和RAR-β2的表达均与宫颈病变程度呈负相关[r=-0.5479(Ach3),r=-0.585(RAR-β2),P〈0.05]。结论:组蛋白乙酰化修饰与RAR-β2表达调控有关,并可能参与宫颈癌的发生。
Objective: To investigate the relationship between cervical lesion development and histone acetylation that regulates RAR-β2 expression. Methods: Immunohistochemistry and Western blot analysis were performed to detect AcH3, RAR-β2, and involu- crin expression in normal cervical tissues as well as in tissues with cervical intraepithelial neoplasia (CIN) Ⅱ-Ⅲ and squamous cell cer-vical carcinoma. The relationship among histone acetylation level, RAR-β2 expression, and cervical lesion severity were analyzed. Re-suits: AcH3, RAR-β2, and involucrin expression were reduced or absent with the progression of cervical lesions; significant differences were noted between the groups (P〈0.05). Histone acetylation level and RAR-132 expression were positively correlated (r=0.797, P〈 0.05). AcH3 and RAR-132 expression, which were both associated with the cervical lesions, were negatively correlated [r=-0.547(AcH3), r=-0.585(RAR-β2), P〈0.05]. Conclusion: Histone acetylation modification is associated with the regulation of RAR-β2 expression. This pro-cess is also likely to participate in the carcinogenesis of cervical carcinoma.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2014年第5期300-304,共5页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金项目(编号:81072127,30901606)
安徽省自然科学基金项目(编号:11040606M176)
安徽医科大学高校科研基金项目(编号:2012XKJ046)资助~~
关键词
组蛋白乙酰化修饰
RAR-β2
宫颈上皮内瘤变
宫颈鳞状细胞癌
分化
histone acetylation modification, RAR-]32, cervical intraepithelial neoplasia, squamous cell cervical carcinoma, differ-entiation