组蛋白乙酰化修饰调控RAR-β2表达与宫颈病变的相关性

Correlation between cervical lesion development and histone acetylation modification that regulates RAR-β_2 expression

目的：探讨组蛋白乙酰化修饰与RAR-β2表达及宫颈病变发生发展的关系。方法：收集正常宫颈、宫颈上皮内瘤变（cervical intraepithelial neoplasia，CIN）Ⅱ-Ⅲ和宫颈鳞状细胞癌（squamous cell carcinoma，SCC）组织，分别采用免疫组织化学和WesternBlot方法检测AcH3、RAR—B：和Involucrin的表达；分析组蛋白乙酰化水平与RAR-β2表达及宫颈病变程度的关系。结果：随着宫颈病变的进展，AcH3、RAR-β2和Involucrin的表达逐渐降低，甚至缺失，组间差异有统计学意义（P〈0．05）；组蛋白乙酰化水平与RAR-β2表达呈正相关（r=0．797，P〈0．05），AcH3和RAR-β2的表达均与宫颈病变程度呈负相关[r=-0．5479（Ach3）,r=-0．585（RAR-β2），P〈0．05]。结论：组蛋白乙酰化修饰与RAR-β2表达调控有关，并可能参与宫颈癌的发生。

Objective： To investigate the relationship between cervical lesion development and histone acetylation that regulates RAR-β2 expression. Methods： Immunohistochemistry and Western blot analysis were performed to detect AcH3, RAR-β2, and involu- crin expression in normal cervical tissues as well as in tissues with cervical intraepithelial neoplasia （CIN） Ⅱ-Ⅲ and squamous cell cer-vical carcinoma. The relationship among histone acetylation level, RAR-β2 expression, and cervical lesion severity were analyzed. Re-suits： AcH3, RAR-β2, and involucrin expression were reduced or absent with the progression of cervical lesions; significant differences were noted between the groups （P〈0.05）. Histone acetylation level and RAR-132 expression were positively correlated （r=0.797, P〈 0.05）. AcH3 and RAR-132 expression, which were both associated with the cervical lesions, were negatively correlated [r=-0.547（AcH3）, r=-0.585（RAR-β2）, P〈0.05]. Conclusion： Histone acetylation modification is associated with the regulation of RAR-β2 expression. This pro-cess is also likely to participate in the carcinogenesis of cervical carcinoma.