摘要
目的探讨Survivin和Caspase-3在胆管细胞癌组织中的表达及其与乙型肝炎病毒(HBV)感染的关系。方法 HE染色对66例检测对象的病理学诊断进行验证;免疫组化检测66例胆管细胞癌中Survivin和Caspase-3的表达,分析其与临床病理参数的关系及二者与HBV感染的关系。结果 66例检测对象均为胆管细胞癌;Survivin和Caspase-3蛋白在胆管细胞癌组织中的阳性表达率分别为73%和60%;Survivin在胆管细胞癌中的表达随着肿瘤分化程度的降低而增强,在血清学HBV阳性肿瘤组织中的表达率显著高于阴性中的表达率(P<0.05);Caspase-3在胆管细胞癌中的表达随着肿瘤分化程度的降低而减弱,在血清学HBV阳性肿瘤组织中的表达率显著低于阴性中的表达率(P<0.05);HBV感染后,Survivin和Caspase-3在胆管细胞癌组织中表达呈负相关(P<0.05)。结论 Survivin和Caspase-3参与胆管细胞癌的发展,但是Survivin不能直接抑制Caspase-3的表达,可能与乙型肝炎病毒相互作用,共同抑制Caspase-3的表达。
Objective To study the expression of Survivin and Caspase-3 in cholangiocarcino- ma tissues and their relevance with hepatitis B virus （HBV） infection. Methods HE staining was used to verify the pathological diagnosis of 66 cases; Immunohistochemistry was used to de- tect the expression of Survivin and Caspase-3 in 66 cholangiocarcinoma. Results Sixty-six ca- ses were all cholangiocarcinoma; The positive expression rates of Survivin and Caspase-3 were 73% and 60% in cholangiocarcinoma, respectively; The expression of Survivin in cholangio- carcinoma tissues increased with the reduction of the differentiation level, and significantly higher in cholangiocarcinoma with HBV infection than that in cholangiocarcinoma without HBV infection （P 〈 0. 05 ） ; The expression of Caspase-3 in cholangicarcinoma tissues decreased with the reduction of the differentiation level, and significantly lower in cholangiocarcinoma with HBV infection than that without （P 〈 0.05 ） ; The expression of Survivin and Caspase-3 incholangiocarcinoma tissues with HBV infection was negatively correlated, the difference was statistically significant （P 〈 O. 05）. Conclusion Survivin and Caspase-3 maybe participate in the occurrence and development of cholangiocarcinoma, but Survivin does not directly inhibit the expression of Caspase-3, it maybe interact with the hepatitis B virus, as a cofactor in inhib- iting the expression of Caspase-3.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2014年第1期25-28,共4页
Journal of Harbin Medical University
