基底前脑巢蛋白免疫反应阳性神经元的来源探索

Exploring of the origination of nestin-immunoreactive neurons in the basal forebrain

目的探索基底前脑巢蛋白免疫阳性神经元的来源,为进一步研究巢蛋白免疫阳性神经元的功能及利用巢蛋白表达改善学习记忆能力提供基础。方法用免疫荧光法研究巢蛋白免疫阳性神经元与5-乙炔基-2’脱氧尿嘧啶核苷(EdU)阳性细胞的关系、用免疫组化法研究巢蛋白免疫阳性神经元与双皮质素阳性神经元的关系。结果 EdU阳性细胞主要分布于海马齿状回及侧脑室的室管膜及室管膜下区的细胞,在内侧隔核、斜角带核等区域无明显的EdU阳性细胞,巢蛋白免疫反应阳性神经元与EdU阳性细胞无共定位表现。双皮质素阳性细胞主要分布于海马齿状回及侧脑室的室管膜及室管膜下区的细胞,巢蛋白免疫反应阳性神经元与双皮质素阳性神经元之间无双标。结论基底前脑巢蛋白免疫反应阳性神经元不是一种新生的神经元,可能是成熟胆碱能神经元的一种功能状态。可能是巢蛋白表达赋予了胆碱能神经元特殊的功能。

Objective To explore the origination of nestin-immunoreactive neurons in the basal forebrain, and provide bases for further functional studying of the nestin-immunoreactive neurons in the basal forebrain and enhancing learning and memory ability by altering nestin expression. Methods Study the relationship of nestin-immunoreactive neurons and 5-ethynyl-2＇-deoxyuri- dine （EdU） positive neurons by immunofluorescence, and study the relationship of nestin-immunoreactive neurons and doubelcortin- immunoreactive neurons using immunohistochemical method. Results EdU positive neurons mainly distribute over dentate gyms of hippocampus, ependyma and subventricular zone of the lateral ventricle, but not over the medial septum, vertical and horizontal limbs of the diagonal band neucleus. No colocalization of nestin-immunoreactive and EdU positive neuron was found. Doublecortin- positive neurons mainly located at dentate gyms of hippocampus, ependyma and subventricular zone of the lateral ventricle and no double staining of nestin-immunoreactive and doublecortin-positive neurons. Conclusion The nestin-immunoreactive neurons in the basal forebrain are not newborn neurons. They may be cholinergic neurons of a special functional stage. Maybe nestin expression gives the cholinergic neurons special features.