栀子中效应成分的稳定性考察及减压提取工艺优选

Investigation of Stability and Optimization of Vacuum Extraction Technology for Active Constituents in Gardeniae Fructus

目的:考察栀子水提液中栀子苷、西红花苷I的稳定性并优选栀子的减压提取工艺参数。方法:采用经典恒温法考察栀子水提液中栀子苷、西红花苷I的稳定性。以栀子苷、西红花苷I含量及浸膏得率为因变量,提取温度、提取时间、料液比为自变量,利用Box-Behnken设计-效应面法优选提取工艺条件。结果:最佳提取工艺参数为提取温度45℃,提取时间60 min,料液比1∶14;栀子苷质量分数3.51%,西红花苷I质量分数1.21%,浸膏得率28.24%,RSD分别为1.44%,2.21%,0.96%,各因变量与预测值偏差较小。结论:减压提取法适合药材中热敏性成分的提取,优选的减压工艺稳定可行,为栀子的综合利用开发提供实验依据。

Objective：To investigate stability of geniposide and crocin-Ⅰ in water extract of Gardeniae Fructus and optimize their vacuum extraction process.Method：Stability of geniposide and crocin-Ⅰ in water extract was studied by classical constant temperature acceleration test.With extract yield,contents of geniposide and crocin-Ⅰ as dependent variables,solid-liquid ratio,extraction temperature and time as independent variables,Box-Behnken design-response surface methodology was optimize vacuum extraction technology.Result：Optimal process parameters was as follows：extraction temperature 45 ℃,extraction time 60 min,ratio of liquid to solid 14∶1 ; Under these conditions,extract yield,contents of geniposide and crocin Ⅰ were 28.24％ （0.96％）,3.51％ （1.44％） and 1.21％ （2.21％）,respectively.Conclusion：Vacuum extraction method was suitable for Chinese herbals which contained heat-sensitive components.This optimized process was reasonable and practicable,which could provide useful reference for comprehensive utilization of Gardeniae Fructus.