星点设计-效应面法优化龙须藤提取工艺

Optimization of Extraction Technology for Bauhinia championi by Central Composite Design-Response Surface Methodology

目的：优选龙须藤的提取工艺.方法：以乙醇体积分数、液料比、提取时间为自变量,浸膏得率、3＇,4＇,5＇,5,7-五甲氧基黄酮（黄酮1）和3 ＇,4＇-亚甲二氧基-5＇,5,6,7-四甲氧基黄酮（黄酮2）含量的总评“归一值”为因变量,通过Design-Expert7.0.0软件对自变量各水平进行多元线性回归和二项式拟合,利用效应面法优选提取工艺并进行预测分析.采用HPLC测定黄酮1和黄酮2含量,色谱条件为流动相乙腈（A）-水（B）洗脱梯度（0～20 min,30％～40％ A;20～36 min,40％ ～48％A）,检测波长324nm.结果：二项式拟合的复合相关系数较高,总评“归一值”达0.995 6;最佳提取工艺为加14倍量45％乙醇提取2次,每次116 min;黄酮1和黄酮2的质量分数依次为0.046％,0.027％,浸膏得率21.21％.结论：利用星点设计-效应面法优化的龙须藤提取工艺稳定简便,具有良好的预测性.

Objective：To optimize extraction process of Bauhinia championi.Method：Taking ethanol concentration,ratio of material to liquid and reflux time as independent variables,overall desirability of dry extract yield,contents of 3＇,4＇,5＇,5,7-pentamethoxyflavone （flavonoids 1） and 3＇,4＇-methylenedioxy-5＇,5,6,7-tetramethoxyflavone （flavonoids 2） as dependent variable,data of overall desirabilities were fitted to a second-order polynomial equation and regressed by multiple linear,response surface methodology was used to optimize extraction technology and predictive analyze.HPLC was adopted to determine contents of flavonoids 1 and flavonoids 2 with mobile phase of acetonitrile （A）-water （B） gradient elution （0-20 min,30％-40％ A; 20-36 min,40％-48％ A） and detection wavelength of 324 nm.Result：Complex correlation coefficient of binomial fitting was higher with overall desirability of 0.995 6 ; Optimal extraction technology was as following：extracted 2 times with 14-fold the amount of 45％ ethanol,116 min per time; The mass fractions of flavonoids 1 and flavonoids 2 were 0.046％ and 0.027％,dry extract yield was 21.21％.Conclusion：Central composite designresponse surface methodology was convenient and highly predictive for optimizing extraction technology of B.championi.