黄连-黄芩药对在泻心汤和葛根芩连汤组方配伍中主要成分的含量变化

Content Change of Main Components of Coptis Rhizome and Scutellaria Radix Couples among Different Compatibilities of Medicinal Materials in Xiexin Decoction and Gegen Qinlian Decoction

目的:探索药对黄连-黄芩在泻心汤和葛根芩连汤组方不同配伍情况下其中所含小檗碱、巴马汀、黄芩苷、黄芩素、汉黄芩素的含量变化。方法:采用HPLC以乙腈-10 mmol·mL-1甲酸铵溶液(内含0.2%三乙胺,用甲酸调pH到4.00)为流动相进行梯度洗脱。结果:组方中黄芩、大黄、甘草使黄连中小檗碱、巴马汀含量降低,葛根使小檗碱、巴马汀含量增加。组方配伍中黄连使黄芩中黄酮类成分含量降低,大黄和甘草对黄芩苷含量的影响无显著性差异,但使黄芩素、汉黄芩素含量降低;葛根使黄芩苷含量降低,但使黄芩素、汉黄芩素含量增加。结论:该方法可以有效鉴定泻心汤和葛根汤中5种成分,不同配伍对5种有效成分含量有影响增加。

Objective：To explore the change of content of berberine,palmatine,baicalin,baicalein,wogonin of and Scutellaria Radix couples among different compatibilities of medicinal materials in Xiexin decoction Coptis Rhizome and and Gegen Qinlian decoction.Method：By HPLC,mobile phase was acetonitrile-0.01 mol ·L-1 ammonium formate （contain 0.2％ triethylamine and adjusted pH to 4.00 with formic acid） in gradient elution.Result：The content of berberine,palmatine of Coptis Rhizome was decreased by Scutellaria Radix,Rhei Radix et Rhizoma and Glycyrrhizae Radix et Rhizoma among different medicinal materials.The content of berberine,palmatine was increased by Puerariae Lobatae Radix.The content of flavonoids of Scutellaria Radix was reduced by Coptis Rhizome.It was insignificant to the difference on the content of baicalin caused by Rhei Radix et Rhizoma and Glycyrrhizae Radix et Rhizoma which decreased the content of baicalein,wogonin.The content of baicalin was reduced by Puerariae Lobatae Radix which caused the increase of content of baicalein and wogonin.Conclusion：Five effective components were influenced by different compatibilities of medicinal materials in Xiexin decoction and Gegen Qinlian decoction.