延龄草根及根茎化学成分研究

Studies on Chemical Constituents of Trillium tschonoskii

目的:对延龄草Trillium tschonoskii Maxim.的化学成分进行研究。方法:采用硅胶柱色谱,大孔树脂,ODS反相柱色谱以及重结晶等方法分离纯化,并通过理化常数和波谱分析鉴定化合物结构。结果:分离并鉴定了10个化合物分别为偏诺皂苷元-3-O-α-L-吡喃鼠李糖基-(1→2)-β-D-葡萄糖苷(1),偏诺皂苷元-3-O-α-L-吡喃鼠李糖基-(1→4)-[α-L-吡喃鼠李糖基-(1→2)]-β-D-葡萄糖苷(2),偏诺皂苷元-3-O-α-L-吡喃鼠李糖基-(1→4)-α-L-吡喃鼠李糖基-(1→4)-[α-L-吡喃鼠李糖基-(1→2)]-β-D-葡萄糖苷(3),豆甾醇(4),β-谷甾醇(5),胡萝卜苷(6),紫云英苷(7),豆甾醇-3-O-β-D-葡萄糖苷-6'-十六烷酸酯(8),豆甾醇-3-O-β-D-葡萄糖苷(9),熊果酸(10)。结论:化合物4,8,9,10为首次从该属植物中分离得到。

Objective：To investigate the chemical compositions of the Trillium tschonoskii.Method：The compounds were isolated and purified from the plant with chromatography techniques and the structures were identified onthe basis of spectral analyses and physicochemical properties.Result：Ten compounds were isolated from T.tschonoskii and their structures were identified as pennogenin-3-O-α-L-rhamnopyranosyl-（1→2）-β-D-glucopyranoside （1）,pennogenin-3-O-α-L-rhamno-pyranosyl-（1 →4） [α-L-rhamnopyranosyl-（1→2）]-β-D-glucopyranoside （2）,pennogenin-3-O-α-L-rhamnopyranosyl-（1→4）-α-L-rhamnopyranosyl-（1→4） [α-L-rhamnopyranosyl-（1→2）]-β-D-glucopyranoside （3）,stigmasterol （4）,β-sitosterol （5）,daucosterol （6）,astragalin （7）,stigmasterol-3-O-β-D-glucopyranoside-6＇-hexacosanoate （8）,stigmasterol-3-O-β-D-glucopyranoside （9）,ursolic acid （10）.Conclusion：Compounds 4,8,9,10 were isolated from Trillium for the first time.