配伍剂量变化对麻杏石甘汤解热作用的影响

Influence of Component Compatibility Changes on Antipyretic Effect of Maxin Shigan Decoction

目的：探讨麻杏石甘汤配伍对其解热作用的影响.方法：利用混料均匀设计,以麻黄（X1）、苦杏仁（X2）、炙甘草（X3）和石膏（X4）4个组分设计4因素12水平的麻杏石甘汤配比实验.将SD健康雄性大鼠随机分为15组,分别为麻杏石甘汤配比N1 ～ N12组、临床常用方组、模型组、空白组.采用尾静脉注射脂多糖（LPS）造模,造模后每0.5h记录1次体温,计算体温反应指数（TRI）;测定造模后6.0h血浆前列腺素E2（PGE2）含量;对结果进行二次多项式回归分析.结果：各配伍组与模型组比较,均能不同程度的抑制LPS诱导的大鼠发热,具有极显著性意义（P＜0.01）;经麻杏石甘汤干预后,血浆PGE2有不同程度的下降;经麻杏石甘汤干预后,TRI有不同程度的下降.回归分析的结果表明,影响TRI的因素为石膏＞麻黄＞苦杏仁与炙甘草的交互作用.麻黄与体温反应指数为负相关.结论：4因素12水平的混料均匀设计实验结果表明：对于疗热,方中石膏为君药,最佳配比剂量为麻黄0.6g,苦杏仁7.5g,甘草7.5g,石膏26.4 g.

Objective：To explore the influence of component compatibility changes on antipyretic effect of Maxin Shigan decoction.Method：The uniform design of experiments with mixtures （UDM） was used,Ephedrae Herba,Armeniacae Semen Amarum,Glycyrrhizae Radix et Rhizoma Preparata Cum Melle and Gypsum Fibrosum were considered as 4 factors,12 different allocated proportions of Maxing Shigan decoction was designed.Rats were randomly divided in to 15 groups,including Maxin Shigan decoction group N1-N12,original group,normal group and model group.The fever model was established by injecting lippolysaccharide （LPS） through the tail vein.The temperature was recoreded once every 0.5 hour,and therml response index （TRI） was calculated.After 6 hours,the plasma concentrations of prostaglandin E2 （PGE2） were detected.The regression analysis was used for data analysis.Result：Compared with the model group,the compatibility group could inhibit the fever induced by LPS,which showed a significant significance （P ＜0.01） ; the decrease of PGE2 levels in plasma and temperature reaction index were found with different degrees.The results of regression analysis showed that the influence of four factors on the TRI was Gypsum Fibrosum ＞ Ephedrae Herba ＞ the interaction of Armeniacae Semen Amarum and Glycyrrhizae Radix et Rhizoma Preparata Cum Melle.Conclusion：The results showed that the influence of Gypsum Fibrosum on the antipyretic effect of Maxin Shigan decoction was most important.The optimal proportion was Ephedrae Herba 0.6 g,Amarum Semen Armeniacae and Glycyrrhizae Radix et Rhizoma Preparata Cum Melle 7.5 g,Gypsum Fibrosum 26.4 g.