4种归肺经中药对肺热证小鼠TNF-α,IL-1β表达的影响

Effects of Four Chinese Herbs Belong to Lung on Expression of TNF-α and IL-1β in Mice with Lung Heat Syndrome

目的:探讨麻黄、干姜、黄芩、桑白皮4味归肺经中药对小鼠肺热证的影响,并初步揭示其作用机制。方法:将100只昆明种小鼠随机分为正常对照组,模型组,麻黄、干姜、黄芩、桑白皮组(20,10 g·kg-1),每组10只,灌胃给药7 d,每天1次,于实验第4,5,6天鼻腔滴入肺炎链球菌菌液建立小鼠肺热证模型,病理学观察肺组织形态变化,酶联免疫吸附法(ELISA)检测血清肿瘤坏死因子-α(TNF-α)含量,免疫组化法测定肺组织TNF-α,白介素-1β(IL-1β)蛋白表达。结果:与正常组比较,模型组肺组织实变(P<0.001),血清TNF-α含量升高(P<0.01),肺组织TNF-α,IL-1β蛋白表达升高(P<0.01)。与模型组比较,麻黄低剂量组,干姜高、低剂量组,黄芩高剂量组肺组织病理有明显改善(P<0.01,P<0.05);血清TNF-α含量降低,除干姜高剂量组、黄芩低剂量组,其余各组差异均有统计学意义(P<0.01或P<0.05);肺组织TNF-α,IL-1β蛋白表达降低,其中,TNF-α表达除干姜低剂量组、桑白皮高剂量组,其余各组差异均有统计学意义(P<0.01或P<0.05),IL-1β表达除麻黄低剂量组、桑白皮低剂量组,其余各组差异均有统计学意义(P<0.01或P<0.05)。结论:麻黄、干姜、黄芩、桑白皮可以干预肺热证,抑制TNF-α,IL-1β炎症因子表达可能是其减轻小鼠肺热证肺组织损伤的主要途径。

Objective：To investigate the effect of four Chinese herbs belong to lung include Ephedra Herba,Zingiberis Rhizoma,Scutellaria Radix and Mori Cortex in mice with lung heat syndrome and to reveal the mechanism preliminarily.Method：Hundred KM mice were randomly divided into ten groups：normal control group,model group,Ephedra Herba,Zingiberis Rhizoma,Scutellaria Radix and Mori Cortex group （20,10 g· kg-1）,10 mice in each group.The four Chinese herbs were administered once a day for seven days,Streptococcus pneumoniae by nose dropping was used to establish lung heat syndrome model at the four,five and six day.The morphological changes of lung tissue were observed,the concentrations of tumor necrosis factor-α （TNF-α） in the serum were measured by enzyme-linked immunosorbant assay （ELISA）,the expressions of TNF-α,interleukin （IL）-1β proteins in lung tissue were analyzed by immunohistochemistry staining.Result：Compared with normal control group,the pathological changes of lung tissue in model group was obvious （P ＜ 0.001）,the concentrations of TNF-α in the serum were higher （P ＜0.01）,the expressions of TNF-α,IL-1β proteins in lung tissue were higher （P ＜ 0.01）.Compared with model group,the pathological changes of lung tissue obviously improved （P ＜ 0.01 or P ＜ 0.05） in Ephedra Herba low dose group,Zingiberis Rhizoma high and low dose groups,Scutellaria Radix high dose group.The concentrations of TNF-α in the serum were decreased,except Zingiberis Rhizoma high dose group and Scutellaria Radix low dose group （P ＜ 0.01,P ＜ 0.05）.The expressions of TNF-α proteins in lung tissue were decreased,except Zingiberis Rhizoma low dose group,Mori Cortex high dose group （P ＜ 0.01 or P ＜ 0.05）.The expressions of IL-1β in lung tissue was decreased （P ＜ 0.01 or P ＜ 0.05） except Ephedra Herba low dose group and Mori Cortex low dose group.Conclusion：Ephedra Herba,Zingiberis Rhizoma,Scutellaria Radix and Mori Cortex can intervene lung heat syndrome,inhibit the expressions of TNF-α,IL-1β inflammatory factor,which may be the main pathwayof reducing lung heat syndrome of lung tissue injury in mice.