二球悬铃木花粉萌发及花粉保存研究

Research on Pollen Germination and Pollen Preservation of Platanus acerifolia

以新鲜采集的二球悬铃木花粉为试材,研究了不同浓度的蔗糖及硼酸对花粉萌发的影响,并在此基础上研究了不同贮藏温度对干燥与未干燥的二球悬铃木花粉生活力的影响。结果表明:在15%蔗糖+0.10%硼酸的液体培养基上花粉萌发最好;不同贮藏温度对花粉的生活力影响显著,随着贮藏温度的降低,花粉生活力上升,且贮藏时间延长,常温条件下贮藏10d后花粉生活力为0%,而在-72℃条件下贮藏38d,干燥花粉仍有24.65%生活力;花粉经硅胶干燥4h后耐贮性明显高于新鲜花粉。综合分析,经硅胶干燥后于-72℃条件下贮藏利于二球悬铃木花粉的保存。

Taking pollen of Platanus acerifolia as material,the effect of different sucrose and boric acid concentration on the pollen germination of Platanus acerifolia was studied,and then the effect of different storage temperature on pollen viability was analyzed. The results showed that pollen had the highest germination rate at the liquid medium with 15% sucrose and 0. 10% boric acid. Storage temperature had a significant effect on pollen viability, with the reduction of storage temperature, the pollen viability risen and extended storage time. At normal temperature,all detected pollen loss viability after 10 days, but it had 24. 65% viability when storage at --72℃ after 38 days. Moreover,the storage property of dry pollen was higher than fresh pollen. In conclusion,dry pollen and storage at --72℃ was favourable for preservation of Platanus aceri folia pollen.