北沙参愈伤组织诱导及悬浮细胞培养研究

Study on Initiation of Callus Tissues and Suspension Cell Culture of Glehnia littoralis

以北沙参叶片和茎段为外植体,采用MS和B5培养基对其进行愈伤组织诱导,继代培养后进行悬浮培养,研究了不同浓度碳源、6-BA和NAA对其细胞生长的影响,以期为进一步建立北沙参细胞培养体系以及提高其次生代谢物产量奠定试验基础。结果表明:1/2MS+0.4mg/L 6-BA+1.5mg/L NAA琼脂培养基为北沙参愈伤组织诱导的较适宜培养基;1/2MS+0.2mg/L 6-BA+1.2mg/L NAA为适宜的继代培养基;在细胞悬浮培养过程中,分别添加0.2mg/L 6-BA、1.6mg/L NAA和20g/L蔗糖有利于细胞的生长。

Taking leaves and stems of Glehnia littoralis as materials,MS and B5 medium were respectively employed to induce the callus of Glehnia littoralis ,subculture after suspension culture,and the effects of different concentrations of carbon source,6-BA and NAA on the growth of the cells were investigated, in order to provide basis for the further establishment of Glehnia littoralis cell culture system and improved the secondary metabolite production. The results showed that the more suitable callus induction of culture medium was 1/2MS＋0. 4 mg/L 6-BA＋1. 5 mg/L NAA;the more suitable subculture medium was 1/2MS＋0. 2 mg/L 6-BA＋1. 2 mg/L NAA;and in the process of cell suspension culture,0. 2 mg/L 6-BA,1.6 mg/L NAA and 20 g/L sucrose would be conducive to cell growth.