超高效液相色谱-串联质谱检测蔬菜中氨基甲酸酯类农药残留

The Inspection of UPLC-MS/MS for the Residue of Carbamate Pesticides in Vegetable

以番茄、茄子、西葫芦、大白菜、甘蓝为试材，采用超高效液相色谱-串联质谱（UP峙MS／MS）技术建立了蔬菜中12种氨基甲酸酯类农药残留的检测方法。结果表明：样品经乙腈提取，氨基固相萃取小柱净化，以watersBEHT3色谱柱分离，采用多反应检测模式对试材进行检测，外标法定量；在质量浓度1～100μg／L范围内呈良好线性，相关系数均大于0．99。在空白基质中添加3个水平加标试验，回收率为65．2％～119．2％，相对标准偏差均小于5％。该方法灵敏度高、准确可靠、精密度好、选择性强，可以满足蔬菜中氨基甲酸酯类农药残留检测的要求。

Ahstract： Taking tomato, eggplant, summer squash, Chinese cabbage and cabbage as materials, twelve carbamate pesticides detecting methods in vegetable was established by utilizing UPLC-MS/IVkS technique. The results showed that the sample was extracted by acetonitri, purified by amino column and separated by water BEH T3 column. The detecting aims were determinate in multiple reaction monitoring（MRM）mode. External standard method was used to determine the residue contents. As a result,the detecting aims were with a good linear relationship（r2〉0. 99）in quality concentration range from 1μg/L to 100 μg/L,and blank sample substrate added to three levels adding standard sample trial,the recowery ranges were from 65. 2% to 119. 2% with the relative standard deviations（RSDs） 5%. The method is highly sensitive and precise and selective,it can be satisfied with those detecting needs for the residue of carbamate pesticide. Key words. UPLC-MS/MS; vegetable; ca rbamate pesticides ; ves tigital