杏鲍菇液体菌种培养基的筛选和优化

Study on the Screening and Optimizing of Liquid Spawn Medium of Pleurotus eryngii

以杏鲍菇为试材,采用摇瓶液体培养法,以菌丝干重、菌球密度、菌丝球回接平板萌发时间和菌球直径为指标,对杏鲍菇液体菌种培养基进行筛选和优化,以期筛选一种适合杏鲍菇生长的液体菌种培养基。结果表明:最佳培养基配方为葡萄糖10g/L,蛋白胨5g/L,黄豆饼粉60g/L,磷酸二氢钾0.5g/L,硫酸镁0.5g/L,维生素B10.01g/L,该配方中菌丝量大、密度高、回接平板萌发时间短,且菌球直径适当。

Taking Pleurotus eryngii as material, the screening and optimizing of liquid spawn medium of Pleurotus eryngii in submerged culture with shake flask were studied according to the indexs of mycelial dry weight, mycelial density, myeelial germination time and the fungus ball diameter. The results showed that the optimum formula for liquid spawn medium of Pleurotus eryngii was glucose 10 g/L,peptone 5 g/L,soybean bread flour 60 g/L,KH2PO4 0. 5 g/L,MgSO4 0. 5 g/L,VI3a 0. 01 g/L, the mycelium of the formula had abundant biomass, high density, short germination time and appropriate diameter.