马铃薯中VIGS沉默体系的研究

The Effects of Different Potato Genotypes and Different Inoculation Methods on VIGS Silence Efficiency

以马铃薯X病毒载体PVX为工具,探究不同马铃薯品种和不同侵染方式对PVX介导的VIGS沉默体系的影响,结果表明:马铃薯基因型的差异是影响VIGS沉默效果的主要因素。通过对VIGS沉默pds(八氢茄红素脱氢酶)基因的植株进行半定量RT-PCR检测,发现在所有供试的马铃薯品种中,乌盟601中pds基因的沉默效果最好,侵染后pds基因表达量下降了79.8%;而虎头、夏坡蒂和大西洋中的pds基因表达量也有不同程度降低,分别为64.1%,31.0%,4.5%。不同侵染方式即叶片注射和茎秆刺伤的试验结果表明,VIGS沉默效果和侵染方式的相关性不显著,采用叶片注射和茎秆刺伤接种马铃薯品种乌盟601,pds基因表达量分别下降了59.0%和56.5%;而虎头叶片注射和茎秆刺伤接种pds基因表达量分别下降了34.7%和40.3%。方差分析结果表明,2种不同的接种方法对pds基因沉默比例没有显著差异。

In this study,factors which affect the silence system of VIGS in potato was unravel,the experiment results suggested that the genotype is the main factor to affect the result of VIGS. Among all the tested potato varie-ties,VIGS showed the highest silence effect in potato variety Wumeng 601,the pds gene expression level was de-cressed by 79. 8%;and the pds gene expression level in Hutou,Shepody and Atlantic was decreased by 64. 1%, 31. 0% and 4. 5% respectively. The effects of inoculation ways such as leaf injection and stem scrub on the silence result of VIGS was also tested,the results suggested that the correlation between inoculation ways and silence effect was not very strong. In Wumeng 601,the pds gene expression level of leaf injection and stem scrub were decreased by 59. 0% and 56. 5% separately;it is 34. 7% and 40. 3% respectively in potato variety Hutou. The analysis of va-riance （ ANOVA） showed there does not have significant difference between the two inoculation ways.