与黄瓜抗黑斑病基因连锁的SCAR标记及抗病资源筛选

SCAR Marker Resistance-related to Cucumber Alternaria Leaf Spot and Resistance Identification of Cucumber Germplasm

为建立黄瓜抗黑斑病分子标记辅助育种体系,以黄瓜感黑斑病母本L 63和抗黑斑病父本L 9及其F1、F2分离群体为试材,将与黑斑病抗性相关基因连锁的一个共显性AFLP标记E-CC/M-CAT进行了测序,根据序列特点设计了特异的SCAR引物SCEM126/122,成功地转换成了简单实用的共显性SCAR标记,经验证该标记与黄瓜抗黑斑病相关基因连锁遗传距离为4.4 cM,可以作为黄瓜抗黑斑病辅助选择的标记。且该标记具有迅速、简便、成本低、不受环境条件限制的优点,扩增条带清晰,无杂带和拖尾现象,适合用于大量样本分析。利用引物SCEM126/122对290份材料进行抗病性检测,结果表明有64份抗病材料,为进行抗黑斑病黄瓜新品种的选育奠定了基础。

In order to establish molecular marker-assisted selection system of cucumber alternaria leaf spot with F1,F2and BC1population between a susceptible parent（L 63） and a resistant parent（L 9）,a co-dominant AFLP marker was successfully converted into a simple, applied and co-dominant SCAR marker. Special marker SCEM 126/122 were designed according to the sequence information of AFLP（ E-CC/M-CAT） fragments. The marker was closely linked to the cucumber alternaria leaf spot resistance-related gene, and the genetic distance between the marker and the gene was 4. 4 cM. The marker could be useful in marker-assisted selection in cucumber breeding. The acquired SCAR marker had many advantages including fast,accurate,low cost,free from an environmental in-fluence et. 64 resistant germplasm were abtained from total 290. Application of the acquired marker will inhance cu-cumber alternaria leaf spot disease resistant germplasm evaluation.