河南省部分地区小麦黑粉病菌的鉴定和系统进化分析

Identification and Phylogenic Analysis of Wheat Smut Fungi of Henan Province

为了对小麦黑粉病及时预报与防治,以采自河南省南阳、周口和平顶山(2个)的4个病原菌菌系为材料,采用传统的形态学鉴定法和ITS序列分析相结合,确定来自南阳和平顶山市区的病原菌菌系为小麦散黑粉菌,来自周口和平顶山郏县的病原菌菌系属于小麦秆黑粉菌。根据ITS序列进行系统进化分析发现,南阳和平顶山市区的散黑粉菌与周口和平顶山郏县的秆黑粉菌分别聚在2个进化枝上。河南省流行的小麦黑粉病可能由散黑粉菌和秆黑粉菌共同导致。

In order to lay the foundation for wheat smut prevention and forecasting,four strains of wheat smut fungi of Nanyang,Zhoukou,Pingdingshan in Henan Province were identified. Internal transcribed spacer （ ITS） ana-lyses and microscopic morphological observations were conducted. It indicated that two strains from Nanyang and Pingdingshan city were Ustilago tritici,another two strains from Zhoukou and Jiaxian of Pingdingshan were Urocystis agropyri. Phylogenetic analysis demonstrated that the strains collected from Nanyang and Pingdingshan districts were clustered into one clade,while other two U. agropyri strains collected from Zhoukou and Pingdingshan Jiaxian were clustered into the other one. The results implied that there was coexistence phenomenon of U. tritici and U. agropyri in Henan Province.