微米大黄炭的质量控制

Study on the quality control of micron Rhubarb charcoal

目的：研究微米大黄炭定性、定量检测方法，建立微米大黄炭的质量控制方法。方法：用HPI。C法测定微米大黄炭中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚含量。采用Fortis universil C18（250mmX4．6mm，5μm）色谱柱，流动相为甲醇（A）-0．2％磷酸溶液（B）进行梯度洗脱，检测波长254nnl，流速1ml·min^-1。结果：芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚分别在0．0848～0．848，0．0896～0．896，0．0896～0．896，0．1244～1．244，0．0914～0．914μg·ml^-1范围内线性关系良好。平均回收率（n=6）分别为98．83％，99．54％，98．99％，99．17％，100．27％；RSD分别为1．05％，0．61％，1．61％，1．83％，1．38％。结论：本方法分析简便、准确，重现性好，可作为该制剂的质量控制。

OBJECtIVE To study the methods of qualitative identification and content determination for micron Rhubarb charcoal and to establish the quality control method of micron Rhubarb charcoal. METHODS The content of aloe-emodin, rheim emodin, chrysophanol and physcin in micron Rhubarb charcoal was determined by HPLC. A Fortis universil C1s column （250 mmX 4. 6 mm,5 μm）was used. The mobile phase was methanol-0. 2%phosphate acid. The detaction wavelength was 254 nm and the flow rate was 1 ml ~ min- 1. RESULTS The linear range was aloe-emodin （0. （784 8 - 0. 848 μg . ml^-1 ）, rhein （0. 089 6 - 0. 896μg . ml^-1 ） ,emodin（0. 089 6 - 0. 896μg . ml^-1 ）, chrysophanol（0. 124 4 - 1. 244μg . ml^-1 and physcin（0. 091 4 - 0. 914μg . ml^-1 ）. The average recovery was 98. 83%, 99. 54%, 98. 99%, 99. 17%, 100. 27% ; with RSD 1.05%, 0. 61%, 1.61%, 1.83 %, 1.38 %respectively. CONCLUSION This method is simple, reliable and accurate, which can be used for quali ty control of micron Rhubarb charcoal.