高效液相色谱法同时测定血尿安胶囊中4种成分含量

Determination of chlorogenic acid,acaciin,ursolic acid and berberine hydrochloride in Xue Niaoan capsule by HPLC at the same tine

目的：建立测定血尿安胶囊中绿原酸、蒙花苷、熊果酸和盐酸小檗碱含量的高效液相色谱法。方法：采用高效液相色谱法，Agilent Extend-C18色谱柱（4．6rain×250mm，5μm）；流动相：甲醇-乙腈-水（含1％冰醋酸）（40：8：52）；流速：1．0ml.min^-1；熊果酸检测波长：215nm；绿原酸、蒙花苷和盐酸小檗碱检测波长：326nm。结果：绿原酸、蒙花苷、熊果酸和盐酸小檗碱线性范围分别为0．05868～O．8803μg、0．3496～5．2436μg、0．05079-0．7619μg、0．2199～3．298 1μg，线性关系良好（r值分别为0．9997、0．9994、0．9995、0．9993），平均回收率分别为97．81％，95．20％，95．49％，97．64％（n=9），RSD分别为0．9％，1．0％，0．8％，0．8％。结论：本方法操作简便，结果准确、可靠。

To establish a method for determination of chlorogenic acid, acaciin, ursolic acid and berberinehydrochloride in Xue Niaoan capsule by HPLC at the same tine. METHODS A Agilent Eclipse XDB-C18 column（4. 6 mm x 250 ram,5 μm） was used. The mobile phase was methanol-acetonitrile-water（including 1% acetic acid glacial）（40：8：52）, the flow rate was 1.0 ml. min^-1, and the detection wavelength was 215 nm for ursolic acid and 326 nm for chlorogenic acid, acaciin and berberine hydrochloride. RESULTS Tile linear range for chlorogenic acid, acaciin, ursolic acid and berberine was 0. 058 68 - 0. 880 μg,0. 349 6- 5. 243 6 μg,0. 050 79 - 0. 761 9 μg,0. 219 9 - 3. 298 1μg （r = 0. 999 7,0. 999 4,0. 999 5,0. 999 3） separately,the average recovery separately was 97. 81%, 95. 20%, 95. 49%, 97. 64% （n = 9）, and RSD also separately was 0. 9%, 1.0% ,0. 8% ,0. 8%. CONCLUSION The method was convenient, accurate and credible.