摘要
目的 探讨协同刺激分子B7-H1、B7-H4和转录因子叉状头/翅膀状螺旋转录因子（Foxp3）在胃癌中的表达及其临床意义.方法 应用免疫组织化学染色法检测B7-H1、B7-H4和Foxp3在100例胃癌组织及癌旁组织中的表达,分析B7-H1、B7-H4和Foxp3的表达与临床病理参数的关系.结果 B7-H1、B7-H4在胃癌组织中高表达,阳性表达率分别为65.0％和71.0％,其表达水平与浸润深度、淋巴结转移及TNM分期有关,差异有统计学意义（P＜0.05）.Foxp3＋调节性T细胞（Treg）在胃癌组织中高表达,并与淋巴结转移有关,差异有统计学意义（P＜0.05）.B7-H1、B7-H4的表达水平和Foxp3＋ Treg细胞数量呈正相关,相关系数分别为：r=0.437,P＜0.05;r=0.456,P＜0.05.B7-H1、B7-H4及Foxp3高表达患者的预后差于低表达者（P＜0.05）.结论 B7-H1、B7-H4和Foxp3＋ Treg细胞在胃癌组织中高表达,并与胃癌患者淋巴结转移和预后有关.
Objective To investigate the expression of B7-H1,B7-H4 and forkhead box P3 （Foxp3） in gastric carcinoma and its clinical significance.Methods The expression of B7-H1,B7-H4 and Foxp3 was detected by using IHC in 100 gastric carcinomas,and the relationship between the expression of B7-H1,B7-H4 and Foxp3 with clinicopathological features was statistically analyzed.Results Both B7-H1 and B7-H4 showed high expression in gastric carcinoma tissues （65.0％ and 71.0％）.The expression of B7-H1 and B7-H4 proteins was positively correlated to the depth of tumor invasion,lymph node metastasis and TNM stage.The positive expression rate of B7-H1 and B7-H4 in the patient with less than 5-year survival was obviously higher than that in the patients with more than 5-year survival （P ＜ 0.05）.High expression of Foxp3 ＋ regulatory T cells （Treg） cells in gastric cancer tissues was correlated to lymph node metastasis （P ＜ 0.05）.Statistical analysis also showed that the expression of B7-H1 and B7-H4 protein was positively correlated to the number of Foxp3 ＋ Treg cells （r =0.437,P ＜ 0.05 ; r =0.456,P ＜0.05）.Conclusion B7-H1,B7-H4 and Foxp3 ＋ Treg cells were up-regulated in gastric cancer tissues,and may be correlated to gastric carcinoma metastasis and prognosis.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第3期492-494,F0003,共4页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（81171653、30972703）
江苏省自然科学基金资助项目（BK2011246、BK2011247）
常州市卫生科技重大项目（ZD201009）
