胃肠Roux-en-Y术后早期胃排空及Cajal间质细胞再生的研究

Regeneration of interstitial cells of Cajal and gastric emptying following Roux-en-Y gastrojejunostomy in rats

目的 探讨大鼠胃空肠Roux-en-Y吻合术后早期胃液体排空功能的变化及胃空肠吻合口Cajal间质细胞（ICC）的再生.方法 20只SD雄性大鼠随机分为实验组和对照组,实验组行幽门切除＋胃空肠Roux-en-Y吻合术,对照组行幽门切断再吻合术.术后4周进行以下实验：放射性核素显像法评估胃液体排空功能;扫描电镜观察胃空肠吻合口ICC再生.结果 术后4周实验组大鼠胃液体半排空时间（t1/2）为（23.5 ±4.5） min,对照组为（10.2±2.3）min,实验组胃液体排空延迟（P＜0.01）;胃空肠吻合口ICC可以再生.结论 大鼠幽门切除＋胃空肠Roux-en-Y吻合术后早期胃液体排空延迟,而胃空肠吻合口ICC再生可能是其重要原因之一.

Objective To investigate the regeneration of interstitial cells of Cajal （ICC） and the gastric emptying in rats undergoing pylorectomy and Roux-en-Y gastrojejunostomy.Methods Twenty male SD rats were divided into two groups randomly：the experimental group and the control group.The experimental group consisted of 10 rats undergoing pylorectomy and Roux-en-Y gastrojejunostomy,and the control group only underwent pyloric transection and anastomosis.Gastric scintigraphy was performed on the two groups respectively to measure the gastric half emptying time （t1/2） at 4th week after the surgical manipulations,and the regeneration of ICC was observed by the scanning electron microscopy.Results The gastric emptying in the experimental group showed a significant delay as compared with that in the control group at 4th week after the surgical manipulations [（23.5 ±4.5） min vs.（10.2 ±2.3） min,P ＜0.01],accompanied with the regeneration of ICC.Conclusion The regeneration of ICC near the gastrojejunal anastomosis may be one of the most important reasons making a significant delay on the gastric emptying.