蛛网膜下腔出血大鼠基底动脉蛋白酶激活受体1和肿瘤坏死因子-α表达变化

The change of expression of protease activated receptor-1 and tumor necrosis factor-α in the basilar artery of rats after subarachnoid hemorrhage

目的 观察大鼠蛛网膜下腔出血（SAH）模型基底动脉蛋白酶激活受体1（PARl）及肿瘤坏死因子-α（TNF-α）表达水平的变化,探讨PAR1、TNF-α与脑血管痉挛（CVS）之间的关系.方法 采用枕大池二次注血的方法,建立大鼠蛛网膜下腔出血后脑血管痉挛模型.实验动物分组：正常组、SAH 3 d组、SAH 5 d组、SAH 7 d组.模型制作成功后灌杀大鼠,取基底动脉标本行组织学检查,计算机软件测量各组大鼠模型脑基底动脉内腔横截面积;免疫组织化学检测各组大鼠模型基底动脉PAR-1、TNF-α表达水平.结果 SAH各时间组模型大鼠基底动脉横截面积较正常组小,随着时间变化痉挛严重程度不同,同时SAH 3 d组、SAH 5 d组及SAH 7 d组基底动脉PAR1与TNF-α表达也呈逐渐增强趋势,差异有统计学意义（P＜0.05）.结论 （1）通过枕大池二次注血法成功建立大鼠SAH模型,基底动脉存在CVS,表现为管壁显著增厚,管腔显著狭窄,该模型可用于对SAH后脑血管疾病的研究平台;（2）经免疫组织化学检测,大鼠模型痉挛的血管壁有PAR1及TNF-α表达上调,并与CVS呈正相关.

Objective To study different expression of protease activated receptor 1 （PAR1） and tumor necrosis factor alpha （TNF-α） in the rat cerebral vasospasm （CVS） model post subarachnoid hemorrhage （SAH）,to discuss the relationship among PAR1,TNF-α,and CVS.Methods The rat cerebral vasospasm models of subarachnoid hemorrhage were made by the method that injection blood into the cisterna magna twice.Experimental groups：normal group,SAH 3 days group,SAH 5 days group,SAH 7 days group.Seven days after the first blood injection,the animals were transcardially perfused with 4％ paraformaldehyde in deep anesthesia and the brain was removed for histological examination.Measure cross-sectional area of vascular changes by computer software; Detect expression level of PAR1 and TNF-α by immunohistochemistry.Results SAH model animals＇ basal artery cross-sectional area was smaller than normal group,as time goes by spasm degree aggravating,the expression level of PAR1 and TNF alpha in SAH 3 d,SAH 5 d and SAH 7 d group tended to increase gradually.Conclusion （1） SAH model was established successfully by injection of autologous blood into the cistern magna,CVS happened in the basilar artery,the wall thickening and the lumen narrowing significantly suggests that the model can be used for researching cerebrovascular disease after SAH; （2） The immunohistochemical detection shows that the expression level of PAR1 and TNF-α increases significantly in the basilar artery of models and has a positive correlation with the degree of CVS.