甲状腺癌发生与EB病毒感染的关系

The relationship between the thyroid carcinoma and epstein-barr virus infection

目的 探讨甲状腺癌发生与EB病毒（EBV）感染的相关性.方法 选取155例甲状腺癌患者手术切除标本为研究对象,根据WHO分类进行分组,并以癌旁正常甲状腺组织为对照,应用免疫组织化学法和原位杂交法检测各组EBV的潜伏膜蛋白-1（LMP-1）和EB病毒基因编码的核糖核酸（EBER-1）的表达.结果 LMP-1和EBER-1在甲状腺癌组织中的阳性表达率分别为41.94％ （65/155）和56.13％（87/155）,明显高于在正常甲状腺组织中的20.00％（31/155）和27.10％ （42/155）,差异均有统计学意义（P＜0.05）.LMP-1和EBER-1在乳头状癌表达阳性率分别为52.13％和60.64％,高于其他病理类型,差异均有统计学意义（P＜0.05）.原位分子杂交技术测定EBER-1的阳性率为56.13％,高于免疫组织化学技术检测LMP-1的阳性率（41.94％）,两种方法检测甲状腺癌组织中EBV感染的结果差异有统计学意义（P＜0.05）,且两种检测方法明显相关（r=0.70）.结论 EBV感染与甲状腺癌的发生发展明显相关,特别是与乳头状癌的关系更为密切.

Objective To discuss the relationship between the thyroid carcinoma and epstein-barr virus （EBV） infection.Methods 155 cases of surgical resection specimen of patients with thyroid cancer were selected as research object,and were divided into groups according to the WHO histological classification,and the pericarcinomatous normal thyroid tissue were selected as the control.Then we applied immunohistochemical method and insitu molecules hybridization to detect the expression of the latent membrane protein-1 （LMP-1） and Epstein-Barr virus encoded RNAs-1 （EBER-1） in the thyroid cancer and cancer adjacent tissues.Results The positive expression rate of LMP-1 and EBER-1 in the thyroid carcinoma tissue were 41.94％ （65/155） and 56.13％ （87/155）,which were significantly higher than in normal thyroid tissue of 20.00％ （31/155） and 27.10％ （42/155）,the difference were statistical significance （P ＜0.05）.And the positive expression rate of LMP-1 and EBER-1 in the papillary carcinoma were 52.13％ and 60.64％,which were higher than other pathological types,the difference were statistical significance （P ＜ 0.05）.The positive expression rate of EBER-1 in the thyroid cancer tissue by the insitu molecules hybridization was 56.13％,which was significantly higher than the positive expression rate of LMP-1 in the thyroid cancer tissue by the immunohistochemical （41.94％）,the difference was statistically significant （P ＜ 0.05）.and the two detection methods had correlation （r =0.70）.Conclusion There has correlation between EBV infection and the occurrence of thyroid cancer development,particularly with papillary carcinoma more closelyd.