SSR标记辅助回交转育大豆7S球蛋白α-亚基致敏蛋白缺失新品系

Soybean 7S( β- conglycinin) α- subunit deficiency lines developed by backcrossing assisted by SSR marker background selection

为快速培育7S球蛋白致敏蛋白α-亚基缺失的优质大豆新品系,以(α'+α)-亚基双缺失型材料日B为供体亲本,东农47为受体亲本,以杂交-回交转育方法为基础,结合SSR标记辅助遗传背景选择与主要农艺性状鉴定及品质分析,在BC2F4选育到7S球蛋白α-亚基缺失新品系Cb80。Cb80综合农艺性状优良,遗传背景回复率大于90%,其蛋白质及氨基酸总量为44.1%、41.95%,分别比轮回亲本东农47提高3.5和3.76个百分点。

Using a Japanese variety RiB with （α＇ ＋ α） -subunits deficiency of 7S globulin as the donor parent, and Dongnong 47 with higher protein and oil content as recurring parent, characteristic of α - subunit deficiency was successfully induced into Dongnong 47 by the methods, which combined with SSR marker assistance and values of agronomic and quality traits based on crossing and backcrossing. A new elite α - null - containing mutant line （Cb80） , showing greater than 90% recurring parent genome content, with higher protein and oil content had been developed in BC2F4. The protein and total amino acids contents of Cb80 were 44.1% and 41.95% , which were higher than the recurring parent Dongnong 47 whose ratios were increased by 3.5% and 3.76% respectively.