肝郁致精瘀对雄性大鼠ACP、E2、α-Glu的影响

Effects of Liver Qi Stagnation Cause Semen Stagnation on ACP、E2 and α-Glu in Male Rats

目的:通过对雄性大鼠慢性束缚刺激的方法建立肝郁精瘀动物模型,探讨肝气郁结与精液瘀阻之间的相关性及内在机理。方法:将8周龄,SD雄性大鼠随机分为对照组(1组)、肝郁药物组(2组)、肝郁精瘀组(3组),每组10只,将2组、3组大鼠进行慢性束缚处理建立肝郁模型。4 w后,观察3组大鼠一般状况及进行旷场试验。第5 w始,对第2组予以柴胡疏肝散汤剂灌胃,第1组和第3组以等体积蒸馏水灌胃(10 mL/kg·d)。于第8 w末次束缚后2 h后处死大鼠,取血清检测ACP、E2、α-Glu,观察其变化。结果:肝郁精瘀组与对照组比较ACP、E2浓度升高,α-Glu浓度下降,肝郁药物组介于2者之间。结论:肝气郁结可通过对ACP、E2、α-Glu的变化而影响生殖之精的正常排泄,致精液瘀阻。提示情志刺激—肝气郁结—精液瘀阻三者之间具有相关性。

Objective： To explore the correlation and the internal mechanism between liver Qi stagnation and semen stasis by creating the semen stagnation animal model. Methods： 8-week-old, SD male rats, all of the rats were randomly divided into three group, such as： control group （first group）, liver Qi stagnation drug group （second group）, semen stasis group （third group）, each group had ten rats, the second group and the third group were chronic restraint to establish liver qi stagnation model. From the fifth week, the second group were given CHSGS gavage processing, the remaining two groups were fed with distilled water （ 10 mL/kg · d）. After 8 weeks, the rats were sacrificed, femoral artery for blood draw, let stand to take the serum detecting the concentration of ACP, E2 and α-Glu. Results： Compared with the semen stasis group and control group, concentrations elevated of ACP and E2, concentrations declined of α-Glu, liver Qi stagnation drug group ACP, E2 and α-Glu concentrations ranged between control group and semen stasis group. Conclusion： Liver Qi stagnation can cause the changes in ACP, E2 and α-Glu, affect the excretion of the semen, caused the semen stasis.