调脂通脉颗粒对ApoE基因敲除小鼠主动脉弓NF-κB、ICAM-1及LFA-1的影响

Influences of Tiaozhi Tongmai Granules on expressions of aortic arch NF-κB,ICAM-1 and LFA-1 in ApoE gene knockout mice

目的探讨调脂通脉颗粒对ApoE基因敲除(ApoE-/-)小鼠主动脉弓核因子-κB(NF-κB)、细胞间黏附分子-1(ICAM-1)及淋巴细胞功能相关抗原-1(LFA-1)的影响。方法 6周龄雄性ApoE-/-小鼠随机分为模型组、血脂康组、调脂通脉颗粒低、中、高剂量组,每组8只,另取C57BL/6J小鼠8只作为正常对照组。高脂饮食结合药物饲养12周,血清学检测血脂变化,主动脉弓根部石蜡切片,HE染色观察组织形态学变化,免疫组化法检测NF-κB、ICAM-1、LFA-1表达。结果与正常对照组比较,模型组、血脂康组、调脂通脉颗粒各组小鼠血清总胆固醇(TC)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)水平均显著升高(P<0.05),模型组、调脂通脉颗粒低剂量组小鼠血清甘油三酯(TG)水平明显升高(P<0.05)。与模型组比较,调脂通脉颗粒中、高剂量组、血脂康组TG水平显著降低(P<0.05)。HE染色表明模型组主动脉内膜增厚伴易损斑块形成,血脂康组及调脂通脉颗粒高剂量组内膜增厚伴稳定斑块形成。免疫组化染色显示,与正常对照组比较,模型组、血脂康组、调脂通脉颗粒各组主动脉弓NF-κB、ICAM-1及LFA-1表达明显升高(P<0.05)。与模型组比较,调脂通脉颗粒高剂量组主动脉弓NF-κB、ICAM-1及LFA-1表达明显降低(P<0.05)。结论调脂通脉颗粒可降低血清TG及主动脉弓NF-κB、ICAM-1及LFA-1的表达,从而减缓动脉粥样病变。

Objective To study the influences of Tiaozhi Tongmai Granules on expressions of aortic arch nuclear factor-κB （NF-KB）, intercellular adhesion molecule-1 （ICAM-1） and lymphocyte function- associated antigen-1 （ LFA-1 ） in apolipoprotein E gene knockout （ ApoE -/- ） mice. Methods Male ApoE-/- mice （6 weeks old） were randomly divided into model group, Xuezhikang group, and low- dose, mid-dose and high-dose Tiaozhi Tongmai high-dose group, each n = 8）. C57BL/6J mice were fed with high fat diet combined with drugs Granules groups （low-dose group, mid-dose group and （n=8） were for 12 weeks. chosen as normal control group. The mice The changes of serum lipid were detectedby using serological test, histomorphological changes of aortic arch root were observed by using HE staining, and expressions of NF-KB, ICAM-1 and LFA-1 were detected by immunohistochemical method. Results The levels of serum total cholesterol （TC）, low density lipoprotein （LDL）, high density lipoprotein （HDL） in model group, Xuezhikang group and Tiaozhi Tongmai Granules groups increased significantly compared with those in control group （ P 〈 0.05 ）. The level of serum triglyceride （TG） increased significantly in model group and low-dose group compared with that in normal control group （P 〈 0.05）. The level of TG decreased significantly in mid-dose group, high-dose group and Xuezhikang group compared with that in model group （P 〈 0. 05 ）. HE staining showed that there were no atherosclerosis plaques formed in aortic intima in control group, yet there were vulnerable atherosclerosis plaques formed in thickened aortic intima in model group, and stable atherosclerosis plaques formed in Xuezhikang group and high-dose group. The expressions of NF-κB, ICAM-1 and LFA-1 increased significantly in model group, Xuezhikang group, low-dose group, mid-dose group and high-dose group compared with those in normal control group （ P 〈 0.05 ）. The expressions of NF-κB, ICAM-1 and LFA- 1 decreased significantly in high-dose group compared with those in model group （P 〈 0. 05 ）. Conclusion Tiaozhi Tongmai Granules can reduce the level of serum TG and expressions of aortic arch NF-κB, ICAM-1 and LFA-1, so as to alleviate artery atherosclerosis lesion.