大黄素对犬自体肾移植缺血再灌注损伤的保护作用研究

Protective effect of emodin on ischemia reperfusion injury of kidney autotransplantation

目的探讨大黄素对犬自体肾移植缺血再灌注损伤的影响及机制。方法将18只健康杂种犬随机分为假手术组、大黄素组和生理盐水组,每组各6只。假手术组仅进行肾手术切除;大黄素组在术前2 h按10 mg/kg ip大黄素,生理盐水组同法使用相同体积的生理盐水。再灌注1 h后检测MDA、MPO、SOD、iNOS、eNOS的水平;术后24 h检测血清肌酐(Cr)、尿素氮(BUN)水平以及TNF-α、IL-6、IL-8、IL-10炎性因子的水平;光镜下观察肾组织病理学改变。结果生理盐水组的MDA、iNOS水平显著高于假手术组,但MPO、SOD、eNOS水平低于假手术组(P<0.05);大黄素组的MDA、iNOS水平显著低于生理盐水组,但MPO、SOD、eNOS水平高于假手术组(P<0.05)。生理盐水组的BUN、Cr、TNF-α、IL-6、IL-8水平显著高于假手术组,但IL-10水平低于假手术组(P<0.05);大黄素组的BUN、Cr、TNF-α、IL-6、IL-8水平显著低于生理盐水组,但IL-10水平高于生理盐水组(P<0.05),肾损伤明显减轻。结论大黄素能减轻肾移植缺血再灌注损伤,其机制可能与减轻肾脂质过氧化反应以及抑制炎性因子有关。

Objective To study the mechanism of emodin on ischemia and reperfusion injury in canine autologous renal transplantation. Methods 18 Healthy mongrel dogs were randomly divided into sham group, emodin group, and normal saline group with 6 dogs in each group. Sham group received kidney operation resection. Dogs in emodin group were injected dosage of 10 mg/kg emodin at 2 h before surgery by intraperitoneal injection, and normal saline group with the method using the same volume. MDA, MPO, SOD, iNOS, and eNOS activity were detected after reperfusion after 1 h, and postoperative serum creatinine （Cr） and blood urea nitrogen （BUN） concentration and the level of TNF- α, IL-6, IL-8, and IL-10 inflammatory factor after 24 h were determined. Renal tissues were observed under light microscope pathological changes. Results The content ofMDA, iNOS, BUN, Cr, TNF-α, IL-6, and IL-8 in normal saline group was significantly higher than that in sham group, while the content of MPO, SOD, eNOS, and IL-10 on the contrary （P〈0.05）. The content of MDA, iNOS, BUN, Cr, TNF-α, IL-6, and IL-8 in emodin group was significantly lower than that in normal saline group, while the contents of MPO, SOD, eNOS, and IL-10 on the contrary （P〈0.05）. Renal injury in emodin group were significantly reduced. Conclusion Emodin can alleviate the ischemia reperfusion injury in renal transplantation, and the mechanism may be related to reduce renal lipid peroxidation and inhibition of inflammatory factor.