长叶胡颓子体外抑制肿瘤细胞增殖的研究

Inhibition of cancer cells proliferation in vitro of Elaeagnus bockii

目的研究长叶胡颓子乙醇提取物、三萜酸部位、熊果酸对4株肿瘤细胞增殖的体外抑制作用以及熊果酸抗肿瘤作用机制。方法采用MTT法测定长叶胡颓子乙醇提取物、三萜酸部位和熊果酸在25、50、100、200μg/mL时对人胃癌细胞SGC-7901、BGC-823、肠癌细胞LOVO、HCT-8实体瘤细胞增殖的体外抑制作用;并在上述药物剂量下,观察熊果酸作用72 h后对SGC-7901细胞形态的影响,以及作用24 h后对SGC-7901细胞凋亡的诱导作用。结果长叶胡颓子乙醇提取物、三萜酸部位、熊果酸对4株肿瘤细胞的增殖具有较强的体外抑制作用,其中对于胃癌细胞的抑制作用为熊果酸>三萜酸部位>乙醇提取物;对肠癌细胞的抑制作用为三萜酸部位>熊果酸>乙醇提取物,同时熊果酸能显著改变SGC-7901细胞的形态,并且SGC-7901细胞的凋亡率与熊果酸的剂量呈相关性。结论三萜酸是长叶胡颓子发挥体外抗肠癌细胞增殖作用的主要有效部位;熊果酸是其发挥体外抗胃癌细胞增殖作用的主要活性成分,熊果酸抑制胃癌细胞增殖机制为诱导细胞凋亡,为开发新的抗癌药物提供参考。

Objective To study the inhibitory effects of alcohol extracts, triterpenic acid fractions, ursolic acid from Elaeagnus bockii on the in vitro proliferation of four cancer cells, and the anticancer mechanism of ursolic acid. Methods The inhibitory effects of the alcohol extracts, triterpenic acid fractions and ursolic acid at the concentration of 25, 50, 100, and 200 μg/mL on human gastric cancer cells SGC-7901, BGC-823, colon cancer cells LOVO, HCT-8 were measured by MTT assay; SGC-7901 cell morphology was observed after treated by ursolic acid for 72 h, and the induction of SGC-7901 cell apoptosis was observed after Ireated by ursolic acid for 24 h. Results The alcohol extracts, triterpenic acid fractions, ursolic acid from E. bockii had the effective inhibitory effects on the in vitro proliferation of four cancer cells. The inhibitory rate of the alcohol extracts, triterpenic acid fractions, and ursolic acid on the proliferation of human gastric cancer cells was ursolic acid 〉 triterpenic acid parts 〉 alcohol extracts; The inhibitiory rate on the proliferation of human intestinal cancer cells was triterpenic acid parts 〉 ursolic acid 〉 alcohol extracts. Ursolic acid could significantly affect the morphology of SGC-7901 cell. The rate of apoptosis markedly elevated along with the increased concentration increasing of ursolic acid. Conclusion Tfiterpenic acid is the main active component on the proliferation of human intestinal cancer cell in vitro. The ursolic acid is the main anticancer effective component on the proliferation of gastric cancer cell in vitro. The anticancer mechanism of ursolic acid is inducing the cell apoptosis, and provides the theoretical basis for new anticancer drug.