HPLC法测定茯苓皮中松苓新酸

Determination of dehydrotrametenolic acid in peels of Poria cocos by HPLC

目的建立茯苓皮中松苓新酸的HPLC测定方法。方法采用高效液相色谱法,Lichrospher C18色谱柱（200 mm×4.6mm,5μm）,流动相为乙腈–0.5%磷酸水溶液（80∶20）,检测波长为242 nm,体积流量为1.0 mL/min,柱温为30℃,进样量为10μL。结果松苓新酸在0.142 5~2.280 0μg与峰面积呈良好的线性关系（r=0.999 3）,平均回收率为101.83%,RSD值为1.45%（n=6）。结论该方法操作简便,稳定可行,重复性好,可用于茯苓皮中松苓新酸的测定。

Objective To establish an HPLC method for the determination of dehydrotrametenolic acid in the peels of Poria cocos. Methods HPLC was carried out on a Lichrospher C18 column （200 mm×4.6 mm, 5 μm） with acetonitrile-0.5% phosphoric acid （80 ： 20） as mobile phase. The detection wavelength was set at 242 nm. The injection volume was 10 gL at the flow rate of 1.0 mL/min. The column temperature was at 30 ℃. Results The calibration curve was linear over the range of 0.142 5--2.280 0μg （r=0.999 3） for dehydrotrametenoli6 acid. The average recovery rate was 101.83% with RSD value at 1.45% （n=6）. Conclusion The established method is rapid, sensitive＇, and accurate, and could be used for the determination of dehydrotrametenolic acid in the peels of e. cocos.