Box-Behnken设计优化铁皮石斛中多糖复合酶法提取工艺

Optimization of Enzymatic Extraction of Polysaccharide from Dendrobium officinale by Box-Behnken Design and Response Surface Methodology

目的:优化复合酶法提取铁皮石斛多糖工艺。方法:采用响应面法,以多糖提取率为评价指标,对复合酶用量、酶解温度、酶解时间三个影响因素进行考察,优化复合酶法铁皮石斛中多糖的提取工艺,另以Design-Expert8.05软件对数据进行综合统计分析。结果:最优工艺为:复合酶量为3.5 mg/mL、酶解温度为53℃、酶解时间为70min,按该工艺进行试验所得铁皮石斛多糖提取率为16.11%。结论:利用Box-Behnken效应面法优选的铁皮石斛多糖复合酶法提取工艺稳定可行,可用于铁皮石斛多糖的提取。

Objective：To optimize the processing of enzymatic extraction of polysaccharide from Dendrobium officinale.Methods：With phenol-sulfuric acid method and the DNS determination polysaccharide,Box-Behnken response surface methodology was used to optimize different enzyme dosage,reaction temperature and reaction time by using Design-Expert 8.05 software for data analysis and processing.Results：According to Box-Behnken response,the best extraction conditions for the polysaccharide from Dendrobium officinale were as follows：the amount of enzyme complex was 3.5 mg/mL,hydrolysis temperature was 53 ℃,and reaction time was 70 min.In accordance with the above process,the polysaccharide yield was 16.11%.Conclusion：Box-Behnken response surface methodology is used to optimize the enzymatic extraction process for the polysaccharide in this study,which is effective,stable and feasible.