丙戊酸钠对人肝癌SMMC-7221细胞的放疗增敏作用研究

Radiosensitization Effect of Valproic Acid on Human Hepatocellular Cell Line SMMC- 7221

目的探讨丙戊酸钠(VPA)对肝癌SMMC-7221细胞的放疗增敏作用及其机制探究。方法 MTT法检测VPA对肝癌SMMC-7221细胞的抑制率,筛选出VPA的半数抑制浓度(IC50)。流式细胞仪(FCM)分析肝癌SMMC-7221细胞周期及细胞凋亡率。结果 MTT结果显示VPA作用SMMC-7221细胞24、48、72 h后IC50分别为41.26、9.04、2.87 mmol/L。FCM结果显示VPA+IR抑制SMMC-7221细胞的增殖,增加凋亡水平和诱导G0/G1期阻滞。结论VPA作为放疗增敏剂,增加了SMMC-7221细胞的杀伤作用,其机制与细胞凋亡和细胞周期阻滞相关。

Objective To investigate radiosensitization effect of valproic acid （VPA） on human hepatocellular cell line SMMC -7221 and its mechanism. Methods MTY was used to detect the proliferation and IC50 of SMMC -7221 cell. FCM was adopted to analyze the changes in cell cycle and apoptosis level. Results After co - cultured with VPA , the IC50 of SMMC -7221 cell was 41.26,9.04,2.87mmol/L. VPA ＋ IR could significantly inhibit the cell proliferation, increase apopto- sis level, cause G0/G1 phase arrest and decrease the decreased the percentages of S phrase. Conclusion VPA could enhance the cell -killing effect of SMMC -7221 cell as a radio - sensitizer, which was associated with apoptosis and cell cycle arrest