含酮替芬的罂粟碱保存液对静脉“桥”血管保护机制的研究

Protective mechanism of papaverine preserving fluid containing ketotifen to vein graft

目的研究含富马酸酮替芬的罂粟碱保存液对冠脉旁路移植术静脉"桥"血管内皮细胞粘附分子-1(ICAM-1)和血管内皮素-1(ET-1)表达的影响。方法收集行冠状动脉旁路移植术(CABG)治疗且大隐静脉(GSV)无病变患者术后剩余GSV20个标本。分别保存在常规罂粟碱液及含富马酸酮替芬的罂粟碱液中各1小时。采用酶联免疫吸附法(ELISA法)检测GSV中ICAM-1、ET-1表达情况。结果含富马酸酮替芬的罂粟碱液组ICAM-1[(32.20±7.70)ng/ml vs.(36.69±5.75)ng/ml]、ET-1[(21.10±7.30)ng/ml vs.(21.69±7.71)ng/ml]表达均低于常规罂粟碱保存液组,差异有统计学意义(P<0.05)。结论体外含富马酸酮替芬的罂粟碱液保存液可以抑制静脉桥ICAM-1、ET-1的表达,有保护静脉桥血管的作用。

Objective To study the influences of papaverine preserving fluid containing ketotifen fumarate on the expressions of vascular endothelial cell adhesion molecule-1 （ICAM-1） and endothelin-1 （ET-1） in vein graft of coronary artery bypass grafting （CABG）. Methods The samples of residual great saphenous vein （GSV, n=20） were collected from the patients treated with CABG and without lesion of GSV, and then the samples were preserved respectively in routine papaverine preserving fluid （group A） and papaverine preserving fluid containing ketotifen fumarate （group B） for 1 h. The expressions of ICAM-1 and ET-1 were detected by using enzyme-linked immunosorbent assay （ELISA）. Results The expressions of ICAM-1 [（32.20±7.70） ng/mL vs. （36.69±5.75） ng/mL] and ET-1 [（21.10±7.30） ng/mL vs. （21.69±7.71） ng/mL] were lower in group B than those in group A （P＆lt;0.05）. Conclusion The papaverine preserving fluid containing ketotifen fumarate in vitro can inhibit the expressions of ICAM-1 and ET-1 and has protective effect on vein graft.